MoCadb-logoMoCadb - Study ID Exp28179577

PubMed ID28179577
AuthorsNishiumi S(1), Kobayashi T(1), Kawana S(2), Unno Y(2), Sakai T(2), Okamoto K(3), Yamada Y(4), Sudo K(4), Yamaji T(5), Saito Y(6), Kanemitsu Y(7), Okita NT(4), Saito H(8), Tsugane S(9), Azuma T(10), Ojima N(2), Yoshida M(10)(1)(11).
TitleInvestigations in the possibility of early detection of colorectal cancer by gas chromatography/triple-quadrupole mass spectrometry.
JournalOncotarget. 2017 Mar 7;8(10):17115-17126.
AbstractIn developed countries, the number of patients with colorectal cancer has been increasing, and colorectal cancer is one of the most common causes of cancer death. To improve the quality of life of colorectal cancer patients, it is necessary to establish novel screening methods that would allow early detection of colorectal cancer. We performed metabolome analysis of a plasma sample set from 282 stage 0/I/II colorectal cancer patients and 291 healthy volunteers using gas chromatography/triple-quadrupole mass spectrometry in an attempt to identify metabolite biomarkers of stage 0/I/II colorectal cancer. The colorectal cancer patients included patients with stage 0 (N=79), I (N=80), and II (N=123) in whom invasion and metastasis were absent. Our analytical system detected 64 metabolites in the plasma samples, and the levels of 29 metabolites differed significantly (Bonferroni-corrected p=0.000781) between the patients and healthy volunteers. Based on these results, a multiple logistic regression analysis of various metabolite biomarkers was carried out, and a stage 0/I/II colorectal cancer prediction model was established. The area under the curve, sensitivity, and specificity values of this model for detecting stage 0/I/II colorectal cancer were 0.996, 99.3%, and 93.8%, respectively. The model's sensitivity and specificity values for each disease stage were >90%, and surprisingly, its sensitivity for stage 0, specificity for stage 0, and sensitivity for stage II disease were all 100%. Our predictive model can aid early detection of colorectal cancer and has potential as a novel screening test for cases of colorectal cancer that do not involve lymph node or distant metastasis.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Homo sapiensbloodplasmaColorectal cancer (CRC)573GC/QqQMS-basedDem28179577a, Dem28179577b

Sample description and preparation

N (case)282
N (control)291
Disease (case)Colorectal cancer (CRC)
Disease (control)healthy

Sample analysis

Software thresholdP<0.05

Molecule list

Molecule IDextExternal IDNameSource accRegulation (case/control)Scores
CHEBI5417 5417glucosamineGlucosamine-5TMS(1)ratio: 0.973
CHEBI13172 13172L-SorboseSorbose-meto-5TMS(1)ratio: 1.57
CHEBI15603 15603L-LeucineLeucine-2TMSratio: 0.901
CHEBI15729 15729L-OrnithineOrnithine-4TMS(/SI)ratio: 1.37
CHEBI15936 15936D-XyloseXylose-meto-4TMS(2)ratio: 1.5
CHEBI16414 16414L-ValineValine-2TMS(/SI)ratio: 0.933
CHEBI16530 165303-methyl-2-oxobutanoate2-keto-isovaleric acid-meto-TMSratio: 0.951
CHEBI16737 16737creatinineCreatinine-3TMSratio: 1.08
CHEBI16828 16828L-TryptophanTryptophan-3TMS(/SI)ratio: 0.895
CHEBI16977 16977L-AlanineAlanine-2TMSratio: 1.1
CHEBI17113 17113erythritolmeso-erythritol-4TMSratio: 1.38
CHEBI17151 17151XylitolXylitol-5TMSratio: 1.23
CHEBI17173 17173D-RibuloseRibulose-meto-4TMSratio: 0.942
CHEBI17203 17203L-ProlineProline-2TMSratio: 1.15
CHEBI17208 17208Ascorbic acidAscorbic acid-4TMSratio: 0.966
CHEBI17301 17301glucaric acidGlucaric acid-6TMSratio: 0.932
CHEBI17306 17306maltoseMaltose-meto-8TMS(1)ratio: 1.07
CHEBI17497 17497Glycol acidGlycolic acid-2TMSratio: 1.34
CHEBI17535 17535L-ArabinoseArabinose-meto-4TMSratio: 1.3
CHEBI17561 17561L-cysteineCysteine-3TMSratio: 0.747
CHEBI17634 17634D-GlucoseGlucose-meto-5TMS(1)ratio: 0.968
CHEBI17775 17775urateUric acid-4TMSratio: 0.85
CHEBI17895 17895L-TyrosineTyrosine-3TMS(/SI)ratio: 0.959
CHEBI17947 179475-Dehydroquinic acid5-dehydroquinic acid-meto-4TMSratio: 0.968
CHEBI17992 17992sucroseSucrose-8TMSratio: 1.53
CHEBI18012 18012Fumaric acidFumaric acid-2TMS(/SI)ratio: 1.4
CHEBI18019 18019L-LysineLysine-4TMSratio: 0.884
CHEBI18333 18333D-arabinitolArabitol-5TMSratio: 1.23
CHEBI26078 26078Phosphoric acidPhosphoric acid-3TMSratio: 0.954
CHEBI27997 279979-Octadecenoic acidElaidic acid-TMSratio: 0.885
CHEBI28716 287169-Hexadecenoic acidPalmitoleic acid-TMSratio: 0.722
CHEBI28757 28757FructoseFructose-meto-5TMS(2)ratio: 1.67
CHEBI30805 30805Lauric acidLauric acid-TMSratio: 0.921
CHEBI30887 30887Isocitric acidIsocitric acid-4TMSratio: 1.17
CHEBI30915 30915Oxoglutaric acid2-ketoglutaric acid-meto-2TMSratio: 1.19
CHEBI32816 32816Pyruvic acidPyruvic acid-meto-TMSratio: 3.02
CHEBI35621 35621Alpha-aminobutyric acid2-aminobutyric acid-2TMSratio: 0.865
CHEBI37084 370843-Hydroxyisovaleric acid3-hydroxyisovaleric acid-2TMSratio: 0.965
CHEBI48300 48300D-threitolThreitol-4TMSratio: 1.21
CHEBI50263 50263Malic acidMalic acid-3TMS(/SI)ratio: 1.21
CHEBI78320 783202-Hydroxypropanoic acidLactic acid-2TMS(/SI)ratio: 1.59

Compile date 10-20-2017© .