MoCadb-logoMoCadb - Study ID Exp27468721

PubMed ID27468721
AuthorsYamamoto T(1), Kudo M(2), Peng WX(2), Takata H(2)(3), Takakura H(1), Teduka K(2), Fujii T(2), Mitamura K(1), Taga A(1), Uchida E(3), Naito Z(4).
TitleIdentification of aldolase A as a potential diagnostic biomarker for colorectal cancer based on proteomic analysis using formalin-fixed paraffin-embedded tissue.
JournalTumour Biol. 2016 Oct;37(10):13595-13606.
AbstractColorectal cancer (CRC) is one of the most common cancers worldwide, and many patients are already at an advanced stage when they are diagnosed. Therefore, novel biomarkers for early detection of colorectal cancer are required. In this study, we performed a global shotgun proteomic analysis using formalin-fixed and paraffin-embedded (FFPE) CRC tissue. We identified 84 candidate proteins whose expression levels were differentially expressed in cancer and non-cancer regions. A label-free semiquantitative method based on spectral counting and gene ontology (GO) analysis led to a total of 21 candidate proteins that could potentially be detected in blood. Validation studies revealed cyclophilin A, annexin A2, and aldolase A mRNA and protein expression levels were significantly higher in cancer regions than in non-cancer regions. Moreover, an in vitro study showed that secretion of aldolase A into the culture medium was clearly suppressed in CRC cells compared to normal colon epithelium. These findings suggest that decreased aldolase A in blood may be a novel biomarker for the early detection of CRC.

Sample characteristics

SpeciesTissue / SourceDiseaseNDetection methodSample
Homo sapienscolon_rectumColorectal cancer (CRC)10LC-MS/MSDem27468721

Sample description and preparation

Disease (case)Colorectal cancer (CRC)
Disease (control)non-cancer regions
Digestin-solution digestion, Glu-C und Trypsin

Sample analysis

Software thresholdP<0.05, fold change>2

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)ValidationScores
A4547 ACTA_HUMANACTA2, ACTSA, ACTVSActin, aortic smooth muscleACTA_HUMAN 
A2341 ACTN1_HUMANACTN1Alpha-actinin 1ACTN1_HUMAN 
A2344 ACTN4_HUMANACTN4Alpha-actinin 4ACTN4_HUMAN 
A8650 AGR2_HUMANAGR2, AG2, UNQ515/PRO1030Secreted cement gland protein XAG-2 homologAGR2_HUMAN 
A1923 ALDOA_HUMANALDOA, ALDAFructose-bisphosphate aldolase AALDOA_HUMAN
A0537 ANXA2_HUMANANXA2, ANX2, ANX2L4Annexin A2ANXA2_HUMAN
A0230 ARPC5_HUMANARPC5, ARC16ARP2/3 complex 16 kDa subunitARPC5_HUMAN 
A0377 ATPA_HUMANATP5A1, ATP5A, ATP5AL2ATP synthase alpha chain, mitochondrial precursorATPA_HUMAN 
A5959 CAH1_HUMANCA1Carbonic anhydrase 1CAH1_HUMAN 
A5964 CAH2_HUMANCA2Carbonic anhydrase 2CAH2_HUMAN 
A6009 CBR1_HUMANCBR1, CBR, CRNCarbonyl reductase [NADPH] 1CBR1_HUMAN 
A4665 CEAM5_HUMANCEACAM5, CEACarcinoembryonic antigen-related cell adhesion molecule 5CEAM5_HUMAN 
A3550 CH60_HUMANHSPD1, HSP6060 kDa heat shock protein, mitochondrial precursorCH60_HUMAN 
A878B CLCA1_HUMANCLCA1, CACC1, HCLCA1Chloride channel, calcium activated, family member 1 precursorCLCA1_HUMAN 
A1491 CO1A1_HUMANCOL1A1Collagen alpha-1(I) chainCO1A1_HUMAN 
A1422 CO6A2_HUMANCOL6A2Collagen alpha 2(VI) chain precursorCO6A2_HUMAN 
A917B CRIP1_HUMANCRIP1, CRIP, CRP1Cysteine-rich protein 1CRIP1_HUMAN 
A9867 DEF1_HUMANEm:AF200455.10, DEFA1, Em:AF200455.12Neutrophil defensin 1 precursorDEF1_HUMAN 
A6319 DHSA_HUMANSDHA, SDH2, SDHFSuccinate dehydrogenase [ubiquinone flavoprotein subunit], mitochondrial precursorDHSA_HUMAN 
A0442 DPYL2_HUMANDPYSL2, CRMP2, ULIP2Dihydropyrimidinase related protein-2DPYL2_HUMAN 
A3684 ECHB_HUMANHADHB, MSTP029Trifunctional enzyme beta subunit, mitochondrial precursorECHB_HUMAN 
A3804 EF2_HUMANEEF2, EF2Elongation factor 2EF2_HUMAN 
A2004 ENPL_HUMANHSP90B1, GRP94, TRA1Endoplasmin precursorENPL_HUMAN 
A9713 FCGBP_HUMANFCGBPIgG Fc binding proteinFCGBP_HUMAN 
A1467 FIBA_HUMANFGAFibrinogen alpha/alpha-E chain precursorFIBA_HUMAN 
A0204 FLNA_HUMANFLNA, FLN, FLN1Filamin AFLNA_HUMAN 
A0331 G3P_HUMANGAPD, GAPDHGlyceraldehyde 3-phosphate dehydrogenaseG3P_HUMAN 
A0534 GFAP_HUMANGFAPGlial fibrillary acidic protein, astrocyteGFAP_HUMAN 
B6500 GLUC_HUMANGCGGlucagon precursorGLUC_HUMAN 
A478B GSLG1_HUMANGLG1, CFR1, ESL1Golgi apparatus protein 1 precursorGSLG1_HUMAN 
A497A H2B1A_HUMANHIST1H2BA, TSH2B, STBPHistone H2B, testisH2B1A_HUMAN 
A499A H2B1C_HUMANHIST1H2BC, HIST1H2BE, HIST1H2BFHistone H2B.a/g/h/kH2B1C_HUMAN 
A1373 H31_HUMANHIST1H3A, H3FA, HIST1H3BHistone H3H31_HUMAN 
A521A H3C_HUMANH3F3CHistone H3.3CH3C_HUMAN 
A558A HNRPL_HUMANHNRNPL, HNRPLHeterogeneous nuclear ribonucleoprotein LHNRPL_HUMAN 
A2008 HS71L_HUMANHSPA1LHeat shock 70 kDa protein 1-HOMHS71L_HUMAN 
A0146 HSP7C_HUMANHSPA8, HSC70, HSP73Heat shock cognate 71 kDa proteinHSP7C_HUMAN 
A3824 K1C10_HUMANKRT10, KPPKeratin, type I cytoskeletal 10K1C10_HUMAN 
A1579 K1C14_HUMANK14, KRT14Keratin, type I cytoskeletal 14K1C14_HUMAN 
A3743 K1C17_HUMANKRT17Keratin, type I cytoskeletal 17K1C17_HUMAN 
A3829 K1C9_HUMANKRT9Keratin, type I cytoskeletal 9K1C9_HUMAN 
A3852 K22E_HUMANKRT2, KRT2A, KRT2EKeratin, type II cytoskeletal 2 epidermalK22E_HUMAN 
A3849 K2C1_HUMANKRT1, KRTAKeratin, type II cytoskeletal 1K2C1_HUMAN 
A4876 K2C1B_HUMANKRT77, KRT1BKeratin 77K2C1B_HUMAN 
A1669 K2C5_HUMANKRT5Keratin, type II cytoskeletal 5K2C5_HUMAN 
A3847 K2C6B_HUMANKRT6B, K6B, KRTL1Keratin, type II cytoskeletal 6BK2C6B_HUMAN 
A3693 KCRB_HUMANCKB, CKBBCreatine kinase B-typeKCRB_HUMAN 
A1506 LAMB3_HUMANLAMB3, LAMNB1Laminin beta-3 chain precursorLAMB3_HUMAN 
A0370 LDHA_HUMANLDHA, PIG19L-lactate dehydrogenase A chainLDHA_HUMAN 
A0370 LDHA_HUMANLDHA, PIG19L-lactate dehydrogenase A chainLDHA_HUMAN 
A1374 LMNA_HUMANLMNA, LMN1Lamin A/CLMNA_HUMAN 
A3696 MDHM_HUMANMDH2Malate dehydrogenase, mitochondrial precursorMDHM_HUMAN 
A7033 MK15_HUMANMAPK15, ERK7, ERK8Mitogen-activated protein kinase 15MK15_HUMAN 
A4992 MUC2_HUMANMUC2, mucin, MLPMucin 2 precursorMUC2_HUMAN 
A3962 MYH14_HUMANMYH14, FP17425Myosin-14MYH14_HUMAN 
A3619 OST48_HUMANDDOST, OST48Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 48 kDa subunit precursorOST48_HUMAN 
A0459 PERI_HUMANPRPH, NEF4, PRPH1PeripherinPERI_HUMAN 
A1555 PGBM_HUMANHSPG2Basement membrane-specific heparan sulfate proteoglycan core proteinPGBM_HUMAN 
A5091 POTEE_HUMANPOTEE, A26C1A, POTE2POTE ankyrin domain family member EPOTEE_HUMAN 
A4369 PPIA_HUMANPPIA, CYPAPeptidyl-prolyl cis-trans isomerase APPIA_HUMAN
A9541 RL12_HUMANRPL1260S ribosomal protein L12RL12_HUMAN 
A242E RL1D1_HUMANRSL1D1, CATX11, CSIGRibosomal L1 domain-containing protein 1RL1D1_HUMAN 
A9561 RL31_HUMANRPL3160S ribosomal protein L31RL31_HUMAN 
A0366 RL7_HUMANRPL7, HUML7-1, RPL7P3260S ribosomal protein L7RL7_HUMAN 
A9640 RS23_HUMANRPS2340S ribosomal protein S23RS23_HUMAN 
A9653 RS7_HUMANRPS740S ribosomal protein S7RS7_HUMAN 
A1550 S10A8_HUMANS100A8, CAGA, CFAGCalgranulin AS10A8_HUMAN 
A1551 S10A9_HUMANS100A9, CAGB, CFAGProtein S100-A9S10A9_HUMAN 
A463C SBP1_HUMANSELENBP1, SBPSelenium-binding protein 1SBP1_HUMAN 
A7801 SETX_HUMANSETX, ALS4, SCAR1Probable helicase senataxinSETX_HUMAN 
A5151 SPTN1_HUMANSPTAN1, SPTA2, NEASSpectrin alpha chain, brainSPTA2_HUMAN 
A371E TAGL2_HUMANTAGLN2, CDABP0035Transgelin 2TAGL2_HUMAN 
A5178 TBA1A_HUMANTUBA1A, TUBA3Tubulin alpha-1A chainTBA1A_HUMAN 
A5188 TBB1_HUMANTUBB1TUBB1 human beta tubulin 1, class VITBB1_HUMAN 
A5193 TBB4A_HUMANTUBB4, TUBB4A, TUBB5Tubulin beta-4 chainTBB5_HUMAN 
A5191 TBB4B_HUMANTUBB2C, TUBB4B, TUBB2Tubulin beta-2 chainTBB2C_HUMAN 
A648C TTHY_HUMANTTR, PALB, TBPATransthyretin precursorTTHY_HUMAN 
A8166 UGGG1_HUMANUGGT1, GT, UGCGL1UDP-glucose:glycoprotein glucosyltransferase 1 precursorUGGG1_HUMAN 
A226B ZHANG_HUMANCREBZF, ZFCREB/ATF BZIP transcription factorZHANG_HUMAN 
A3258 ZN408_HUMANZNF408, PFM14, PRDM17Zinc finger protein 408ZN408_HUMAN 
A0467 1433B_HUMANYWHAB14-3-3 protein beta/alpha1433B_HUMAN 
A550A HNRCL_HUMANHNRPCL1, HNRNPCL1Heterogeneous nuclear ribonucleoprotein C-like 1HNRCL_HUMAN 
BJ860 RS30_HUMANFAU40S ribosomal protein S30RS30_HUMAN 
BJ900 SP20H_HUMANSUPT20H, FAM48A, FP757Protein FAM48AFA48A_HUMAN 

Compile date 10-20-2017© .