MoCadb-logoMoCadb - Study ID Exp24424155

PubMed ID24424155
AuthorsPhua LC(1), Chue XP(1), Koh PK(2), Cheah PY(3), Ho HK(1), Chan EC(1).
TitleNon-invasive fecal metabonomic detection of colorectal cancer.
JournalCancer Biol Ther. 2014 Apr;15(4):389-97.
AbstractColorectal cancer (CRC) is a major cause of mortality in many developed countries. Effective screening strategies were called for to facilitate timely detection and to promote a better clinical outcome. In this study, the role of fecal metabonomics in the non-invasive detection of CRC was investigated. Gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) was utilized for the metabolic profiling of feces obtained from 11 CRC patients and 10 healthy subjects. Concurrently, matched tumor and normal mucosae surgically excised from CRC patients were profiled. CRC patients were differentiated clearly from healthy subjects based on their fecal metabonomic profiles (orthogonal partial least squares discriminant analysis [OPLS-DA], 1 predictive and 3 Y-orthogonal components, R (2)X = 0.373, R (2)Y = 0.995, Q (2) [cumulative] = 0.215). The robustness of the OPLS-DA model was demonstrated by an area of 1 under the receiver operator characteristic curve. OPLS-DA revealed fecal marker metabolites (e.g., fructose, linoleic acid, and nicotinic acid) that provided novel insights into the tumorigenesis of CRC. Interestingly, a disparate set of CRC-related metabolic aberrations occurred at the tissue level, implying the contribution of processes beyond the direct shedding of tumor cells to the fecal metabotype. In summary, this work established proof-of-principle for GC/TOFMS-based fecal metabonomic detection of CRC and offered new perspectives on the underlying mechanisms.

Sample characteristics

SpeciesTissue / SourceDiseaseNDetection methodSample
Homo sapienscolon_rectumColorectal cancer (CRC)11GC/TOFMSDem24424155

Sample description and preparation

Disease (case)Colorectal cancer (CRC)
Disease (control)matched normal mucosae

Sample analysis

Software thresholdP<0.05

Molecule list

Molecule IDextExternal IDNameSource accRegulation (case/control)Scores
CHEBI16704 16704uridineUridineratio: 3.21p-value: <0.05
CHEBI16737 16737creatinineCreatinineratio: 0.49p-value: <0.05
CHEBI17050 170503-phosphoglycerate3-phosphoglycerateratio: 0.32p-value: <0.05
CHEBI17203 17203L-ProlineProlineratio: 2.12p-value: <0.05
CHEBI17568 17568uracilUracilratio: 4.06p-value: <0.05
CHEBI17596 17596InosineInosineratio: 0.46p-value: <0.05
CHEBI17634 17634D-GlucoseGlucoseratio: 0.1p-value: <0.05
CHEBI28061 28061D-GalactoseGalactoseratio: 0.11p-value: <0.05
CHEBI30769 30769citric acidCitric acidratio: 0.35p-value: <0.05

Compile date 10-20-2017© .