MoCadb-logoMoCadb - Study ID Exp24138801

PubMed ID24138801
AuthorsWang H, Wang L, Zhang H, Deng P, Chen J, Zhou B, Hu J, Zou J, Lu W, Xiang P, Wu T, Shao X, Li Y, Zhou Z(1), Zhao YL.
Title1H NMR-based metabolic profiling of human rectal cancer tissue.
JournalMol Cancer. 2013 Oct 18;12(1):121.
AbstractBACKGROUND: Rectal cancer is one of the most prevalent tumor types. Understanding the metabolic profile of rectal cancer is important for developing therapeutic approaches and molecular diagnosis. METHODS: Here, we report a metabonomics profiling of tissue samples on a large cohort of human rectal cancer subjects (n = 127) and normal controls (n = 43) using 1H nuclear magnetic resonance (1H NMR) based metabonomics assay, which is a highly sensitive and non-destructive method for the biomarker identification in biological systems. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and orthogonal projection to latent structure with discriminant analysis (OPLS-DA) were applied to analyze the 1H-NMR profiling data to identify the distinguishing metabolites of rectal cancer. RESULTS: Excellent separation was obtained and distinguishing metabolites were observed among the different stages of rectal cancer tissues (stage I = 35; stage II = 37; stage III = 37 and stage IV = 18) and normal controls. A total of 38 differential metabolites were identified, 16 of which were closely correlated with the stage of rectal cancer. The up-regulation of 10 metabolites, including lactate, threonine, acetate, glutathione, uracil, succinate, serine, formate, lysine and tyrosine, were detected in the cancer tissues. On the other hand, 6 metabolites, including myo-inositol, taurine, phosphocreatine, creatine, betaine and dimethylglycine were decreased in cancer tissues. These modified metabolites revealed disturbance of energy, amino acids, ketone body and choline metabolism, which may be correlated with the progression of human rectal cancer. CONCLUSION: Our findings firstly identify the distinguishing metabolites in different stages of rectal cancer tissues, indicating possibility of the attribution of metabolites disturbance to the progression of rectal cancer. The altered metabolites may be as potential biomarkers, which would provide a promising molecular diagnostic approach for clinical diagnosis of human rectal cancer. The role and underlying mechanism of metabolites in rectal cancer progression are worth being further investigated.

Sample characteristics

SpeciesTissue / SourceDiseaseNDetection methodSample
Homo sapiensrectumRectal cancer (RC)1271H nuclear magnetic resonance (1H NMR)Dem24138801

Sample description and preparation

Disease (case)Rectal cancer (RC)
Disease (control)adjacent normal-appearing tissue

Sample analysis

Software thresholdP<0.05

Molecule list

Molecule IDextExternal IDNameSource accRegulation (case/control)Scores
CHEBI15344 15344acetoacetic acidAcetoacetateratio: 1.21
CHEBI15366 15366acetic acidAcetateratio: 2.97
CHEBI15444 15444D-Glucose?-Glucoseratio: -3.495
CHEBI15603 15603L-LeucineLeucineratio: 1.17
CHEBI15611 15611sarcosineSarcosineratio: 1.02
CHEBI15724 15724Trimethylamine oxideTrimethylamine-N-oxideratio: 2.96
CHEBI15741 15741succinic acidSuccinateratio: 1.84
CHEBI15891 15891taurineTaurineratio: -2.1
CHEBI16830 16830methamineMethylamineratio: 1.3
CHEBI16856 16856glutathioneGlutathioneratio: 1.415
CHEBI16857 16857L-threonineThreonineratio: 1.14
CHEBI16919 16919creatineCreatineratio: -2.12
CHEBI17115 17115L-SerineSerineratio: 1.45
CHEBI17170 17170N-MethylmethamineDimethylamineratio: 1.28
CHEBI17268 17268myo-inositolMyo-inositolratio: -2.07
CHEBI17287 17287N-phosphocreatinePhosphocreatineratio: -2.12
CHEBI17568 17568uracilUracilratio: 3.195
CHEBI17724 17724N,N-DimethylglycineDimethylglycineratio: -2.38
CHEBI17750 17750glycine betaineBetaineratio: -1.89
CHEBI17895 17895L-TyrosineTyrosineratio: -1.36
CHEBI18019 18019L-LysineLysineratio: 1.35
CHEBI18050 18050L-GlutamineGlutamineratio: 1.4
CHEBI18132 18132phosphocholinePC(phosphochline)ratio: 1.22
CHEBI20067 200673-Hydroxybutyric acid?-hydroxybutyrateratio: 1.545
CHEBI24364 24364glyceryl groupGlycerylratio: -1.45
CHEBI30751 30751Formic acidFormateratio: 1.43
CHEBI78320 783202-Hydroxypropanoic acidLactateratio: 1.52

Compile date 10-20-2017© .