MoCadb-logoMoCadb - Study ID Exp22395091a

PubMed ID22395091
AuthorsMa Y(1), Zhang P, Wang F, Liu W, Yang J, Qin H.
TitleAn integrated proteomics and metabolomics approach for defining oncofetal biomarkers in the colorectal cancer.
JournalAnn Surg. 2012 Apr;255(4):720-30.
AbstractOBJECTIVE: The present study was designed to search for potential diagnostic biomarkers in the serum of colorectal cancer (CRC). BACKGROUND: CRC is the third most common cancer worldwide, and its prognosis is poor at early stages. A panel of novel biomarkers is urgently needed for early diagnosis of CRC. METHODS: An integrated proteomics and metabolomics approach was performed to define oncofetal biomarkers in CRC by protein and metabolite profiling of serum samples from CRC patients, healthy control adults, and fetus. The differentially expressed proteins were identified by a 2-D DIGE (2-Dimensional Difference Gel Electrophoresis) coupled with a Finnigan LTQ-based proteomics approach. Meanwhile, the serum metabolome was analyzed using gas chromatography-mass spectrometry integrated with a commercial mass spectral library for peak identification. RESULTS: Of the 28 identified proteins and the 34 analyzed metabolites, only 5 protein spots and 6 metabolites were significantly increased or decreased in both CRC and fetal serum groups compared with the healthy adult group. Data from supervised predictive models allowed a separation of 93.5% of CRC patients from the healthy controls using the 6 metabolites. Finally, correlation analysis was applied to establish quantitative linkages between the 5 individual metabolite 3-hydroxybutyric acid, L-valine, L-threonine, 1-deoxyglucose, and glycine and the 5 individual proteins MACF1, APOH, A2M, IGL@, and VDB. Furthermore, 10 potential oncofetal biomarkers were characterized and their potential for CRC diagnosis was validated. CONCLUSION: The integrated approach we developed will promote the translation of biomarkers with clinical value into routine clinical practice.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Homo sapiensbloodserumColorectal cancer (CRC)60GC-MSDem22395091a, Dem22395091b

Sample description and preparation

N (case)30
N (control)30
Disease (case)Colorectal cancer (CRC)
Disease (control)healthy

Sample analysis

Software analysis toolDeCyder software
Software thresholdP<0.05

Molecule list

Molecule IDextExternal IDNameSource accRegulation (case/control)Scores
CHEBI15428 15428glycineGlycineratio: 0.713461538
CHEBI15963 15963D-ribitolRibitolratio: 0.657794677
CHEBI16070 160701,5-anhydro-D-glucitol1-Deoxyglucoseratio: 0.668451172
CHEBI16414 16414L-ValineL-Valineratio: 0.745646766
CHEBI16857 16857L-threonineL-Threonineratio: 0.556379822
CHEBI20067 200673-hydroxybutyric acid3-Hydroxybutyric acidratio: 2.100961538

Compile date 10-20-2017© .