PADB-logoLSSR - dataset 18440303

PubMed ID18440303
AuthorsWang Y, Chen Y, Zhang Y, Wu S, Ma S, Hu S, Zhang L, Shao C, Li M, Gao Y.
TitleDifferential ConA-enriched urinary proteome in rat experimental glomerular diseases.
JournalBiochem Biophys Res Commun. 2008 Jul 4;371(3):385-90. Epub 2008 Apr 25
AbstractGlomerular diseases are leading causes of end-stage renal diseases worldwide. They are considered to be consequences of injury primarily to the three types of glomerular cells. Differential diagnosis typically relies on invasive biopsy findings. We expected that injuries of different glomerular cells would cause different changes in urinary proteome. The goal of this study was to identify differential urinary proteins distinguishing between injuries of different glomerular cells before significant histopathologic changes. Adriamycin nephropathy and Thy1.1 glomerulonephritis were employed as models with different primary impaired cells. ConA-enriched urinary glycoproteome on day3 were profiled by gel-free shotgun tandem mass spectrometry, and compared with self-healthy controls to identify differential urinary proteins for each model. By comparing the changes of the differential proteins between these two models, we identified 39 proteins with different directions of changes, which may potentially be useful in differentiation; and 7 proteins with the same direction of changes, which may be potential indicators of early renal damage. These differential proteins were of several origins: plasma proteins, proteins with urine or kidney specificity, proteins without tissue-specificity (mainly inflammatory mediators) etc. Our results may help better understand the effects of injuries of different glomerular cells at the initial stage, and lead to the discovery of novel early diagnostic markers for human focal segmental glomerulosclerosis (FSGS) and mesangioproliferative glomerulonephritis (MsPGN) which have the same primary impaired cells with adriamycin nephropathy and Thy1.1 glomerulonephritis, respectively.

SpeciesTissue / SourceSubcellDiseaseSample digestSeparationDetectionQuantification
raturineglycoproteinsglomerular disease/adriamycin nephropathy and Thy1.1 glomerulonephritisPNGase F, trypsin, in-solutionConALC-MS/MSspectral counting (subtraction of pre- and post disease induction)

Identified molecules

Molecule IDReferenceGene/SymbolNameSource acc#Regulation in diseaseRatio (disease/control)Frequency % found in diseaseFrequency % found in healthyAssociated diseaseBiomarkerNID stats
A021CHEMO_HUMANHpxHemopexin precursorIPI00195516 1.8168.2937.8  20
A0318CADH1_HUMANCdh1Epithelial-cadherin precursorIPI00206662 0.7340.655.64  20
A0326EZRI_HUMANVil2EzrinIPI00470254 2.7510036.36  20
A0353SPA3L_RATLOC299282Serine protease inhibitor A3L precursorIPI00200591 >1001000  20
A0419GELS_HUMANGsnGelsolin precursorIPI00363974 1.5010066.67  20
A0433TPIS_HUMANTpi1Triosephosphate isomeraseIPI00231767 0.000100  20
A0504INAR2_HUMANLOC686326similar to Interferon-alpha/beta receptor beta chain precursorIPI00766451 0.000100  20
A0609EGF_HUMANEgfPro-epidermal growth factor precursorIPI00231848 0.6737.0855.06  20
A0821CD44_HUMANCd44CD44 proteinIPI00195685 0.065.3691.07  20
A0821CD44_HUMANCd44Isoform 2 of CD44 antigen precursorIPI00230935 >1001000  20
A1001DNAS1_HUMANDnase1Deoxyribonuclease-1 precursorIPI00230876 1.8081.8245.45  20
A127ASFRP4_HUMANSfrp4Secreted frizzled-related protein 4 precursorIPI00207235 0.3030100  20
A1322PIGR_HUMANPigrPolymeric-immunoglobulin receptor precursorIPI00205255 2.0090.9145.45  20
A1423CO6A3_HUMANCol6a3predicted similar to alpha 3 type VI collagen isoform 2 precursorIPI00360737 0.3126.0985.51  20
A1466FINC_HUMANFn1Isoform 1 of Fibronectin precursorIPI00200757 1.6710060  20
A1468PLMN_HUMANPlgPlasminogen precursorIPI00206780 0.3316.6750  20
A1469THRB_HUMANF2Prothrombin precursor (Fragment)IPI00189981 0.7877.78100  20
A1471VTNC_HUMANVtnAa1018IPI00210120 0.5044.4488.89  20
A149CMUP_RATLOC259246Major urinary protein precursorIPI00191711 0.7764.1283.21  20
A149CMUP_RATLOC298111Alpha2u globulinIPI00200422 0.00062.5  20
A149CMUP_RATMup4Alpha-2u globulinIPI00201596 >1001000  20
A149CMUP_RATLOC259246;LOC259245Alpha-2u globulinIPI00201616 >1001000  20
A149CMUP_RATObp3;LOC259244Alpha-2u globulinIPI00201617 0.000100  20
A149CMUP_RATLOC298109;LOC259246Alpha-2u globulinIPI00392753 >1001000  20
A149CMUP_RAT 11 kDa proteinIPI00559211 >1001000  20
A149CMUP_RAT Alpha-2u globulinIPI00563352 >1001000  20
A149CMUP_RAT 17 kDa proteinIPI00567122 >1001000  20
A149CMUP_RATLOC682694similar to alpha2u globulinIPI00767078 >1001000  20
A149CMUP_RATLOC366380similar to alpha-2u globulin PGCL2IPI00776965 >1001000  20
A1555PGBM_HUMANHspg2284 kDa proteinIPI00778528 0.000100  20
A1564VCAM1_HUMANVcam1Vascular cell adhesion protein 1 precursorIPI00205631 0.5050100  20
A1581ALBU_HUMANAlbSerum albumin precursorIPI00191737 1.8148.3326.77  20
A1595CO4_RATC4aComplement C4 precursorIPI00213036 0.000100  20
A1602CFAB_HUMANCfbDa1-24IPI00382185 >1001000  20
A1602CFAB_HUMANCfbB-factor, properdinIPI00422011 0.2215.6371.88  20
A1610LRP2_HUMANLrp2Low-density lipoprotein receptor-related protein 2 precursorIPI00205325 0.756080  20
A1614DAF_HUMANDaf1Decay accelerating factor soluble-form precursorIPI00207872 1.5355.3236.17  20
A1620CFAD_HUMANCfdComplement factor D precursorIPI00212480 >1001000  20
A1630CD59_HUMANCd59CD59 glycoprotein precursorIPI00195173 0.000100  20
A1631HPT_HUMANHpIsoform 2 of Haptoglobin precursorIPI00382202 >10042.860  20
A1669K2C5_HUMANKrt2-5Keratin, type II cytoskeletal 5IPI00382153 0.000100  20
A1939IGHG1_HUMANIGH, VH1, IGHG115 kDa proteinIPI00389939 2.2982.0535.9  20
A2407COCA1_HUMANCol12a1similar to Collagen alpha-1(XII) chain precursorIPI00189714 0.000100  20
A2505TAF4B_HUMANRGD1562997predicted similar to Transcription initiation factor TFIID 105 kDa subunitIPI00763237 >1001000  20
A278D LOC691352;LOC686820similar to Robo-1IPI00769183 1.8970.8337.5  20
A279D  53 kDa proteinIPI00475984 0.7572.7396.97  20
A280D LOC688253similar to Urinary protein 3 precursorIPI00763182 0.6945.4565.91  20
A307ESG2A2_HUMANScgb2a2Secretoglobin family 2A member 2 precursorIPI00191771 0.8351.161.89  20
A315BBAMBI_HUMANBambiBMP and activin membrane-bound inhibitor homolog precursorIPI00207960 0.5757.14100  20
A337ESPBP_RATSbpProstatic spermine-binding protein precursorIPI00213958 >10089.580  20
A379CS12A7_HUMANSlc12a7similar to Solute carrier family 12 member 7IPI00358860 0.000100  20
A4212SAP_HUMANPsapSulfated glycoprotein 1 precursorIPI00195160 1.1173.9166.67  20
A421AFA98B_HUMANRGD1564603predicted similar to family with sequence similarity 98, member BIPI00565909 0.8066.6783.33  20
A4552ACTG_HUMANActg1similar to Actin, cytoplasmic 2IPI00764461 0.5641.6775  20
A472EUP1_RAT Urinary protein 1 precursorIPI00205273 0.4034.4186.02  20
A473EUP2_RAT Urinary protein 2 precursorIPI00205275 0.033.1493.08  20
A474EUP3_RATLOC680367Urinary protein 3 precursorIPI00205971 0.000100  20
A4751DSC2_HUMANDsc2Desmocollin 2IPI00358704 1.005050  20
A4769DYH7_HUMANDnah7similar to axonemal dynein heavy chain 7IPI00767028 2.0010050  20
A4793ESAM_HUMANEsamEndothelial cell-selective adhesion molecule precursorIPI00207475 0.000100  20
A4857IBP7_HUMANIgfbp7Insulin-like growth factor binding protein 7IPI00361798 0.6754.5581.82  20
A4952LY6D_HUMANLy6dpredicted similar to Lymphocyte antigen Ly-6D precursorIPI00361103 0.000100  20
A4994MXRA8_HUMANMxra8Matrix-remodelling associated 8IPI00196249 0.000100  20
A5080PIP_HUMANPipProlactin-inducible protein homolog precursorIPI00198494 1.3873.3353.33  20
A5209TFF1_HUMANTff1Trefoil factor 1 precursorIPI00209094 0.9548.6551.35  20
A5210TFF3_HUMANTff3Trefoil factor 3 precursorIPI00208027 0.6751.5676.56  20
A5298CRIS1_HUMANCrisp1Cysteine-rich secretory protein 1 precursorIPI00193789 >1001000  20
A5314DLK1_HUMANDlk1ZOG proteinIPI00388901 0.000100  20
A5419NPC2_HUMANNpc2Epididymal secretory protein 1 precursorIPI00193171 0.5736.3663.64  20
A5701ACV1B_HUMANAcvr1bActivin receptor type-1B precursorIPI00195456 0.000100  20
A5802AMY1_HUMANAmy1Alpha-amylaseIPI00198466 0.9055.3561.64  20
A5923BGAL_HUMANGlb1predicted similar to Beta-galactosidase precursorIPI00371132 >1001000  20
A5925BGLR_HUMANGusbBeta-glucuronidase precursorIPI00206623 >1001000  20
A5982CATB_HUMANCtsbCathepsin B precursorIPI00212811 0.5033.3366.67  20
A6042CERU_HUMANCpGPI-anchored ceruloplasminIPI00325847 >10066.670  20
A6196CP7B1_HUMANCyp7b1similar to cytochrome P450, family 7, subfamily b, polypeptide 1IPI00373174 2.0066.6733.33  20
A6216CSF1R_HUMANCsf1rCsf1r proteinIPI00656391 0.2525100  20
A6348DNMT1_HUMANDnmt1Isoform 1 of DNA (cytosine-5)-methyltransferase 1IPI00327802 0.000100  20
A6365DPP2_HUMANDpp7Dipeptidyl-peptidase 2 precursorIPI00230946 2.2091.6741.67  20
A6413ECP1_MOUSEEar1Ribonuclease 16IPI00553856 0.000100  20
A643CTRFE_HUMANTfIsoform 1 of Serotransferrin precursorIPI00679202 5.6454.449.65  20
A6589GGH_HUMANGghGamma-glutamyl hydrolase precursorIPI00209265 1.6010062.5  20
A6594GILT_HUMANIfi30Interferon gamma inducible protein 30IPI00196145 0.000100  20
A6853KLK1_HUMANNgfgnerve growth factor, gammaIPI00231193 0.9459.2662.96  20
A6854KLK10_HUMANKlk1c10;Klk10similar to Glandular kallikrein-10 precursorIPI00766750 0.5334.7365.27  20
A6862KLK7_HUMANKlk7predicted Glandular kallikrein-7, submandibular/renal precursorIPI00201592 5.3384.215.8  20
A6864KLK9_HUMANKlks3Submandibular glandular kallikrein-9 precursorIPI00209517 2.1662.7429  20
A6935LYSC_HUMANLyz;LOC688047Putative lysozyme C type 2 precursorIPI00193370 0.6438.8961.11  20
A6997MEP1A_HUMANMep1aMeprin A subunit alpha precursorIPI00210872 1.005050  20
A7111NAPSA_HUMANNapsaNapsinIPI00212697 4.7510021.05  20
A7149NEP_HUMANMmeNeprilysinIPI00231789 0.000100  20
A734AMUG1_MOUSEMug1Isoform 1 of Murinoglobulin-1 precursorIPI00212666 8.0010012.5  20
A742CA2GL_HUMANLrg1Leucine-rich alpha-2-glycoprotein 1IPI00393521 3.0010033.33  20
A7540PTGDS_HUMANPtgdsProstaglandin-H2 D-isomerase precursorIPI00203102 0.5033.3366.67  20
A760BYIPF3_HUMANYipf3Protein YIPF3IPI00197444 1.5274.5149.02  20
A7653QSOX1_HUMANQscn6Isoform 1 of Sulfhydryl oxidase 1 precursorIPI00200685 0.5753.3393.33  20
A7671REG3G_HUMANReg3gRegenerating islet-derived protein 3 gamma precursorIPI00200614 1.1942.2235.56  20
A7693RISC_HUMANScpep1Retinoid-inducible serine carboxypeptidase precursorIPI00200344 0.674060  20
A7710RNAS2_HUMANLOC474169Pre-eosinophil-associated ribonuclease-2IPI00206658 0.000100  20
A7724RNS1G_RATRGD1565230predicted Ribonuclease pancreatic gamma-type precursorIPI00202434 0.000100  20
A7842SODC_HUMANSod1Superoxide dismutaseIPI00231643 0.6046.9978.31  20
A7843SODE_HUMANSod3Extracellular superoxide dismutase [Cu-Zn] precursorIPI00200507 0.000100  20
A790DPBAS_RATPbsnIsoform 1 of Probasin precursorIPI00208410 1.005050  20
A795BAFAM_HUMANAfmAfamin precursorIPI00207668 1.2273.3360  20
A7971TGM4_HUMANTgm4Protein-glutamine gamma-glutamyltransferase 4IPI00197575 1.3810072.73  20
A8021TRAF7_HUMANTraf7predicted similar to ring finger and WD repeat domain 1 isoform 1IPI00372335 2.5781.8231.82  20
A8273KV402_HUMANLOC500180Ig kappa chain C region, B alleleIPI00855219 0.9945.7746.27  20
A8273KV402_HUMANLOC500183Ig kappa chain C region, A alleleIPI00188967 0.000100  20
A8364LV605_HUMANIGL, IGLV3-22, V2-15Ig lambda-2 chain C regionIPI00370486 1.448559  20
A8364LV605_HUMANIGL, IGLV3-22, V2-1513 kDa proteinIPI00557253 0.000100  20
A8364LV605_HUMANIGL, IGLV3-22, V2-1515 kDa proteinIPI00557663 >1001000  20
A8364LV605_HUMANIGL, IGLV3-22, V2-1514 kDa proteinIPI00563097 0.000100  20
A8364LV605_HUMANIGL, IGLV3-22, V2-1515 kDa proteinIPI00565413 1.6710060  20
A8364LV605_HUMANIGL, IGLV3-22, V2-1514 kDa proteinIPI00568091 0.7758.6275.86  20
A836722P1_RATAndproCystatin-related protein 1 precursorIPI00205208 3.2783.0825.37  20
A836822P2_RATP22k15Cystatin-related protein 2 precursorIPI00205210 0.2719.2872.29  20
A836822P2_RATRGD1563136predicted similar to Cystatin-related protein 2 precursorIPI00373145 3.5984.7223.61  20
A8369A1AT_HUMANSerpina1Alpha-1-antiproteinase precursorIPI00324019 >10070.450  20
A8383AMBP_HUMANAmbpAMBP protein precursorIPI00210900 0.7346.1562.82  20
A8401CYTC_HUMANCst3Cystatin-C precursorIPI00231801 0.6747.2770.91  20
A8412EXPI_RATExpiExtracellular peptidase inhibitorIPI00779816 0.8637.543.75  20
A8427ISK1_HUMANSpink3Serine protease inhibitor Kazal-type 3 precursorIPI00324027 0.8080100  20
A8444OVOS_MOUSERGD1565709predicted similar to cDNA sequence BC048546IPI00358444 4.2010023.81  20
A844DPSC1_RATScgb1d2Prostatic steroid-binding protein C1 precursorIPI00191774 1.0852.8849.04  20
A845DPSC2_RATPsbpc2Prostatic steroid-binding protein C2 precursorIPI00191773 1.2857.4944.95  20
A8492SPA3K_MOUSESerpina3kSerine protease inhibitor A3K precursorIPI00200593 2.1838.117.46  20
A8492SPA3K_MOUSESerpina3kserine protease inhibitor 2bIPI00782664 0.8994.23105.77  20
A8527WFDC2_HUMANWfdc2Isoform 1 of WAP four-disulfide core domain protein 2 precursorIPI00193169 0.5542.8678.57  20
A8528WFDC3_HUMANWfdc3predicted similar to WAP four-disulfide core domain 3 isoform 1 precursorIPI00373360 1.7553.8530.77  20
A8663APOH_HUMANApohBeta-2-glycoprotein 1 precursorIPI00195241 0.000100  20
A8663APOH_HUMANApohApolipoprotein HIPI00778633 0.1412.587.5  20
A870D PRP-2similar to CG7896-PA isoform 1IPI00768108 >1001000  20
A871D LOC296235Similar to Cystatin SIPI00373146 2.1868.5431.46  20
A8774FBLN3_HUMANEfemp1EGF-containing fibulin-like extracellular matrix protein 1 precursorIPI00365221 0.000100  20
A8783FETUA_HUMANAhsgAlpha-2-HS-glycoprotein precursorIPI00327469 1.1760.8752.17  20
A894D Fcgbpl1RGD1311906 protein (Fragment)IPI00364039 0.9163.7570  20
A9038SAP3_HUMANGm2aGM2 ganglioside activator proteinIPI00470260 1.4462.943.55  20
A9106THIO_HUMANTxn1ThioredoxinIPI00231368 0.000100  20
A9139UROM_HUMANUmodUromodulin precursorIPI00198021 0.9375.2581.19  20
A913D Muc10Apomucin precursorIPI00206980 0.000100  20
A9149VTDB_HUMANGcVitamin D-binding protein precursorIPI00194097 0.9242.8646.43  20
A914D LOC171161Common salivary protein 1 precursorIPI00204777 1.0552.5350.13  20
A915D PRP-2Proline-rich protein (Fragment)IPI00655260 4.008020  20
A9271CLC4A_HUMANClecsf6C-type lectin domain family 4 member AIPI00393396 2.0073.9136.96  20
A9312GFRA3_HUMANGfra3Glial cell line derived neurotrophic factor family receptor alpha 3IPI00464517 0.000100  20
A9357IL1R2_HUMANIl1r2Interleukin-1 receptor type II precursorIPI00199341 0.000100  20
A9399LYVE1_HUMANXlkd1predicted similar to extra cellular link domain-containing 1IPI00359024 0.000100  20
A9881DFB50_RATDefb50Beta-defensin 50IPI00558849 0.000100  20
A9929GRN_HUMANGrnGranulins precursorIPI00213847 0.2826.3294.74  20
A9934HEPC_HUMANHampHepcidin precursorIPI00197915 0.000100  20
A9938ICOSL_HUMANRGD1562791predicted similar to ICOS ligand precursorIPI00391338 0.000100  20
A9969KNT1_RATKng1T-kininogen 1 precursorIPI00327182 >10067.740  20

Compile date 12-23-2014© PADB initiative