cvd-logocvd - Study ID Exp21235297

PubMed ID21235297
AuthorsLiu D, Glaser AP, Patibandla S, Blum A, Munson PJ, McCoy JP, Raghavachari N, Cannon RO.
TitleTranscriptional profiling of CD133(+) cells in coronary artery disease and effects of exercise on gene expression.
JournalCytotherapy. 2011 Feb;13(2):227-36.
AbstractBACKGROUND AIMS: Bone marrow (BM)-derived progenitor cells are under investigation for cardiovascular repair but may be altered by disease. Our aim was to identify differences in gene expression in CD133(+) cells of patients with coronary artery disease (CAD) and healthy controls, and determine whether exercise modifies gene expression. METHODS: CD133(+) cells were flow-sorted from 10 CAD patients and four controls, and total RNA was isolated for microarray-based gene expression profiling. Genes that were found to be differentially regulated in patients were analyzed further to investigate whether exercise had any normalizing effect on CD133(+) cells in CAD patients following 3 months of an exercise program. RESULTS: Improvement in effort tolerance and increases in the number of CD133(+) cells were observed in CAD patients after 3 months of exercise. Gene expression analysis of the CD133(+) cells identified 82 differentially expressed genes (2-fold cut-off, 25% false-discovery rate and % present calls) in patients compared with controls, of which 59 were found to be up-regulated and 23 down-regulated. These genes were found to be involved in carbohydrate metabolism, cell cycle, cellular development and signaling, and molecular transport. Following completion of the exercise program, gene expression patterns resembled those of controls in seven of 10 patients. CONCLUSIONS: Alterations in gene expression of BM-derived CD133(+) progenitor cells were found in CAD patients, which in part may be normalized by exercise.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Homo sapiensbone_marrowCD133(+) cellsCAD14Gene-arrayDem21235297

Sample description and preparation

N (case)10
N (control)4
Disease (case)Coronary artery disease (CAD)
Disease (control)healthy controls

Sample detection

Array versionAffymetrix Human Genome U133 Plus 2.0 Array

Sample analysis

Software thresholdFDR<0.25, fold change>2

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)Scores
A3405 HXA9_HUMANHOXA-9, HOXA9, HOXA10Homeobox protein Hox-A9209905_atratio: 19.040p-value: <0.05
A068B T22D1_HUMANTSC22D1, TGFB1I4, TSC22TSC22 domain family protein 1215111_s_atratio: 11.000p-value: <0.05
A167G DPOLB_HUMANPOLBDNA polymerase beta203616_atratio: 4.720p-value: <0.05
A073G DBR1_HUMANDBR1Lariat debranching enzyme234295_atratio: 3.880p-value: <0.05
A498B L37A2_HUMANLRRC37A2Leucine-rich repeat-containing protein 37A2221740_x_atratio: 3.640p-value: <0.05
A8996 RBGP1_HUMANRABGAP1, HSPC094, TBC1D11Rab GTPase-activating protein 1213313_atratio: 3.620p-value: <0.05
A9802 BST2_HUMANBST2Bone marrow stromal antigen 2201641_atratio: 3.430p-value: <0.05
A564E BAP29_HUMANBCAP29, BAP29B-cell receptor-associated protein 29225674_atratio: 3.380p-value: <0.05
A5546 FBX7_HUMANFBXO7, FBX7F-box only protein 71554423_a_atratio: 3.200p-value: <0.05
A287E SCOC_HUMANSCOC, SCOCOShort coiled-coil protein SCOCO224786_atratio: 3.190p-value: <0.05
A4231 STAG1_HUMANSTAG1, SA1Cohesin subunit SA-1202293_atratio: 3.180p-value: <0.05
A6692 HCD2_HUMANHSD17B10, ERAB, HADH23-hydroxyacyl-CoA dehydrogenase type 2202282_atratio: 3.080p-value: <0.05
A0845 STAT3_HUMANSTAT3, APRFSignal transducer and activator of transcription 3208991_atratio: 3.030p-value: <0.05
A174F 5HT1F_HUMANHTR1F, HTR1EL5-hydroxytryptamine 1F receptor221458_atratio: 2.960p-value: <0.05
A0311 KPCI_HUMANPRKCI, DXS1179EProtein kinase C, iota type213518_atratio: 2.960p-value: <0.05
A772G HINFP_HUMANHINFP, MIZF, ZNF743Histone H4 transcription factor206495_s_atratio: 2.930p-value: <0.05
A0643XPARVB_HUMANPARVB, CGI-56, CLINTBeta-parvin37966_atratio: 2.920p-value: <0.05
A475G FNIP2_HUMANFNIP2, FNIPL, MAPO1Folliculin-interacting protein 2225922_atratio: 2.920p-value: <0.05
A1325 AP3M1_HUMANAP3M1Adapter-related protein complex 3 mu 1 subunit222516_atratio: 2.880p-value: <0.05
B7206 HMGN4_HUMANHMGN4, HMG17L3, NHCNonhistone chromosomal protein HMG-17-like 3209786_atratio: 2.840p-value: <0.05
B1N20 100132884 Conserved hypothetical protein228899_atratio: 2.830p-value: <0.05
A0306 KAP3_HUMANPRKAR2BcAMP-dependent protein kinase type II-beta regulatory chain203680_atratio: 2.800p-value: <0.05
A1462 HAT1_HUMANHAT1, KAT1Histone acetyltransferase 1203138_atratio: 2.770p-value: <0.05
A615A IREB2_HUMANIREB2Iron-responsive element-binding protein 2225892_atratio: 2.750p-value: <0.05
A0275 CRIPT_HUMANCRIPT, HSPC139Cysteine-rich PDZ-binding protein227942_s_atratio: 2.730p-value: <0.05
A4398 TIM10_HUMANTIMM10, TIM10Mitochondrial import inner membrane translocase subunit TIM10218408_atratio: 2.710p-value: <0.05
A495A H2AZ_HUMANH2AFZ, H2AZHistone H2A.z200853_atratio: 2.680p-value: <0.05
A494H NARF_HUMANNARFNuclear Prelamin A recognition factor219862_s_atratio: 2.660p-value: <0.05
A991C CCD58_HUMANCCDC58Coiled-coil domain-containing protein 58235244_atratio: 2.650p-value: <0.05
A347F APC13_HUMANANAPC13Anaphase-promotingcomplex subunit 13209001_s_atratio: 2.650p-value: <0.05
A3569 PSMD8_HUMANPSMD826S proteasome non-ATPase regulatory subunit 8200820_atratio: 2.630p-value: <0.05
A7421 PLCD_HUMANAGPAT4, UNQ499/PRO1016, UNQ4991-acylglycerol-3-phosphate O- acyltransferase 4228667_atratio: 2.630p-value: <0.05
A0858 RIPK1_HUMANRIPK1, RIP, RIP1Receptor-interacting serine/threonine protein kinase 1226551_atratio: 2.590p-value: <0.05
A0275 CRIPT_HUMANCRIPT, HSPC139Cysteine-rich PDZ-binding protein222702_x_atratio: 2.580p-value: <0.05
A8947 PRAF1_HUMANRABAC1, PRA1, PRAF1Prenylated Rab acceptor protein 1203136_atratio: 2.580p-value: <0.05
A4167 NEDD1_HUMANNEDD1Neural cell expressed, developmentally down-regulated gene 1234984_atratio: 2.560p-value: <0.05
A050D UQCC2_HUMANUQCC2, MNF1Uncharacterized protein224448_s_atratio: 2.530p-value: <0.05
A434F ATGA1_HUMANATG101, PP894Autophagy-related protein 101218214_atratio: 2.520p-value: <0.05
A4296 DJC14_HUMANDNAJC14, DRIP78, HDJ3DnaJ homolog subfamily C member 14224914_s_atratio: 2.480p-value: <0.05
A469B GIMA2_HUMANGIMAP2, IMAP2, HIMAP2GTPase IMAP family member 2232024_atratio: 2.440p-value: <0.05
A2997 ZN213_HUMANZNF213, ZKSCAN21Zinc finger protein 213227207_x_atratio: 2.420p-value: <0.05
A8817 GOLP3_HUMANGOLPH3, GPP34Golgi phosphoprotein 3217803_atratio: 2.410p-value: <0.05
A6848 KGUA_HUMANGUK1, GMKGuanylate kinase 1200075_s_atratio: 2.390p-value: <0.05
A8969 PSMD4_HUMANPSMD4, MCB126S proteasome non-ATPase regulatory subunit 4210460_s_atratio: 2.380p-value: <0.05
A962C CC127_HUMANCCDC127Coiled-coil domain-containing protein 127226515_atratio: 2.380p-value: <0.05
A341G F172A_HUMANFAM172AUPF0528 protein FAM172A212936_atratio: 2.370p-value: <0.05
A1326 VPS41_HUMANVPS41, HVPS41Vacuolar protein sorting-associated protein 4 homolog203106_s_atratio: 2.360p-value: <0.05
A9968 KLHL6_HUMANKLHL6Kelch-like protein 6228167_atratio: 2.290p-value: <0.05
A4376 PPIL3_HUMANPPIL3Cyclophilin-like protein PPIL3b224364_s_atratio: 2.280p-value: <0.05
A7444 PMM1_HUMANPMM1, PMMH22Phosphomannomutase 1203467_atratio: 2.280p-value: <0.05
A970G JKAMP_HUMANJKAMP, JAMP, CDA06JNK1/MAPK8-associated membrane protein223215_s_atratio: 2.220p-value: <0.05
A9752 SPD2A_HUMANSH3PXD2A, FISH, SH3MD1SH3 multiple domains 1224817_atratio: 2.210p-value: <0.05
A820K WDR17_HUMANWDR17WD repeat-containing protein 171557132_atratio: 2.210p-value: <0.05
A5790 AMFR_HUMANAMFR, RNF45E3 ubiquitin-protein ligase AMFR202204_s_atratio: 2.200p-value: <0.05
A0195 INSR_HUMANINSRInsulin receptor226450_atratio: 2.170p-value: <0.05
B1962 CARD8_HUMANCARD8, NDPP1, TUCANCaspase recruitment domain protein 81554479_a_atratio: 2.150p-value: <0.05
A0377 ATPA_HUMANATP5A1, ATP5A, ATP5AL2ATP synthase alpha chain, mitochondrial precursor213738_s_atratio: 2.100p-value: <0.05
A869A PQBP1_HUMANPQBP1, NPW38, JM26Polyglutamine binding protein 1214527_s_atratio: 2.090p-value: <0.05
BO322 B7Z294HERC2P2-locus, MN7, IRX1HERC2P2 protein217317_s_atratio: 2.020p-value: <0.05
A3789 MCAF1_HUMANATF7IP, MCAF, MCAF1Activating transcription factor 7-interacting protein 1231825_x_atratio: -2.070p-value: <0.05
BA833 NPIB5_HUMANNPIPB5Nuclear pore complex-interacting protein family member B5235060_atratio: -2.180p-value: <0.05
A692G GPTC2_HUMANGPATC2, GPATCH2G patch domain containing protein 2242224_atratio: -2.220p-value: <0.05
A256G ELP2_HUMANELP2, STATIP1STAT3-interacting protein235623_atratio: -2.290p-value: <0.05
A2382 OPHN1_HUMANOPHN1Oligophrenin 1206323_x_atratio: -2.370p-value: <0.05
A0373 ADA22_HUMANADAM22, MDC2ADAM 22 precursor1555024_atratio: -2.370p-value: <0.05
A199B ZC3H1_HUMANZFC3H1, PSRC2, CCDC131Zinc finger C3H1 domain-containing protein213065_atratio: -2.410p-value: <0.05
A8976 PTMA_HUMANPTMA, PTMAP7, TMSAProthymosin alpha200772_x_atratio: -2.430p-value: <0.05
A1727 M3K9_HUMANMAP3K9, MLK1, PRKE1Mitogen-activated protein kinase kinase kinase 9214969_atratio: -2.530p-value: <0.05
A998B GGA2_HUMANGGA2ADP-ribosylation factor binding protein GGA2208913_atratio: -2.530p-value: <0.05
A1049 SP100_HUMANSP100Nuclear autoantigen Sp-100202864_s_atratio: -2.560p-value: <0.05
A062D MASU1_HUMANMALSU1IOJAP-related protein family protein244641_atratio: -2.600p-value: <0.05
A847D PSG4_HUMANPSG4, CGM4Pregnancy-specific beta-1-glycoprotein 4208191_x_atratio: -2.670p-value: <0.05
A7866 SRPK2_HUMANSRPK2Serine/arginine-rich protein-specific kinase 2203181_x_atratio: -2.710p-value: <0.05
A8976 PTMA_HUMANPTMA, PTMAP7, TMSAProthymosin alpha208549_x_atratio: -2.730p-value: <0.05
A573E DKK1_HUMANDKK1, UNQ492/PRO1008, UNQ492Dickkopf related protein-1 precursor204602_atratio: -2.750p-value: <0.05
A0307 KPCA_HUMANPRKCA, PKCA, PRKACAProtein kinase C alpha type213093_atratio: -2.940p-value: <0.05
A8976 PTMA_HUMANPTMA, PTMAP7, TMSAProthymosin alpha211921_x_atratio: -3.010p-value: <0.05
A051J TYSY_HUMANTYMS, TSThymidylate synthase1554696_s_atratio: -3.070p-value: <0.05
A545J RBM6_HUMANRBM6, DEF3, NY-LU-12RNA-binding protein 6230742_atratio: -3.470p-value: <0.05
A468D FNBP4_HUMANFNBP4, FBP30Formin-binding protein 4229272_atratio: -4.550p-value: <0.05

Compile date 02-09-2018© C/VD project