CKDdb-logoCKDdb - Study ID Exp24223694

PubMed ID24223694
AuthorsBarutta F, Tricarico M, Corbelli A, Annaratone L, Pinach S, Grimaldi S, Bruno G, Cimino D, Taverna D, Deregibus MC, Rastaldi MP, Perin PC, Gruden G.
TitleUrinary exosomal microRNAs in incipient diabetic nephropathy.
JournalPLoS One. 2013 Nov 4;8(11):e73798.
AbstractMicroRNAs (miRNAs), a class of small non-protein-encoding RNAs, regulate gene expression via suppression of target mRNAs. MiRNAs are present in body fluids in a remarkable stable form as packaged in microvesicles of endocytic origin, named exosomes. In the present study, we have assessed miRNA expression in urinary exosomes from type 1 diabetic patients with and without incipient diabetic nephropathy. Results showed that miR-130a and miR-145 were enriched, while miR-155 and miR-424 reduced in urinary exosomes from patients with microalbuminuria. Similarly, in an animal model of early experimental diabetic nephropathy, urinary exosomal miR-145 levels were increased and this was paralleled by miR-145 overexpression within the glomeruli. Exposure of cultured mesangial cells to high glucose increased miR-145 content in both mesangial cells and mesangial cells-derived exosomes, providing a potential mechanism for diabetes-induced miR-145 overexpression. In conclusion, urinary exosomal miRNA content is altered in type 1 diabetic patients with incipient diabetic nephropathy and miR-145 may represent a novel candidate biomarker/player in the complication.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Homo sapiensurineexosomesDiabetes22RT-PCRDem24223694

Sample description and preparation

N (case)12
N (control)10
Disease (case)type 1 diabetes (w/ microalbuminuria)
Disease (control)healthy
Preparation specific methodtotal RNA extraction, Trizol reagent

Sample detection

Array versionHuman TaqMan miRNA Array A

Sample analysis

Software thresholdfold change >2, P<0.05

Molecule list

Molecule IDextExternal IDNameRegulation (case/control)Scores
AZ0H6 MI0000461hsa-mir-145quantification method: qRT-PCR
AZ0H0 MI0000448hsa-mir-130aquantification method: qRT-PCR
AZ0CA MI0000681hsa-mir-155quantification method: qRT-PCR
AZ14Y MI0001446hsa-mir-424quantification method: qRT-PCR

Compile date 08-10-2018© iMODE-CKD consortium