CKDdb-logoCKDdb - Study ID Exp24096133c

PubMed ID24096133
AuthorsCaseiro A, Barros A, Ferreira R, Padrao A, Aroso M, Quintaneiro C, Pereira A, Marinheiro R, Vitorino R, Amado F.
TitlePursuing type 1 diabetes mellitus and related complications through urinary proteomics.
JournalTransl Res. 2014 Mar;163(3):188-99.
AbstractDiabetes mellitus is a chronic metabolic disease with multiple complications, and its successful management requires early diagnosis, to allow timely interventions. Here, we have comprehensively analyzed the proteome changes in urine of type 1 diabetic subjects with and without complications such as retinopathy and nephropathy. gel electrophoresis combined to liquid chromatography-tandem mass spectrometry (GeLC-MS/MS) analysis of midstream urine highlighted the mechanisms involved in disease pathogenesis as, for instance wound healing and blood coagulation in all diabetics or altered ganglioside metabolism in retinopathy, and also some urinary proteins with potential diagnosis value. From these, gelsolin and antithrombin-III appear as promising diagnosis markers for type 1 diabetes mellitus (T1DM), whereas ephrin type-B receptor 4 and vitamin K-dependent protein Z seem to be promising markers for advanced T1DM disease state presenting retinopathy and nephropathy (T1DM-R + N). Data also suggest urinary ganglioside GM2 activator and beta-hexosaminidase subunit beta as potential urinary markers of retinopathy in diabetics. Taken together, the present exploratory urinary proteomic analysis might be seen as an important starting point for studies targeting specific urinary proteins aimed at the implementation of new biomarkers for the early detection of T1DM-related microvascular complications.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Homo sapiensurinewholeDiabetes10MALDIDem24096133a, Dem24096133d

Sample description and preparation

N (case)5
N (control)5
Disease (case)Diabetes type 1 with Retinopathy and Nephropathy
Disease (control)healthy
Preparation specific method10 kDa Vivaspin, 1DE
Digesttrypsin

Sample analysis

Software thresholdfold change > 2

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)Scores
A1177 A2MG_HUMANA2M, CPAMD5, FWP007Alpha-2-macroglobulin precursorA2MG_HUMANratio: 1000
A5647XABHEB_HUMANABHD14B, CIBAlpha/beta hydrolase domain-containing protein 14BABHEB_HUMANratio: 0
A4547 ACTA_HUMANACTA2, ACTSA, ACTVSActin, aortic smooth muscleACTA_HUMANratio: 1000
A4549 ACTBL_HUMANACTBL2Beta-actin-like protein 2ACTBL_HUMANratio: 0
A3494 AGRIN_HUMANAGRN, AGRINAgrinAGRIN_HUMANratio: 0.5
A1581XALBU_HUMANALB, GIG20, GIG42Serum albumin precursorALBU_HUMANratio: 3.99
A5800 AMPN_HUMANCD13, ANPEP, APNAminopeptidase NAMPN_HUMANratio: 3.25
A1702XANGT_HUMANAGT, SERPINA8AngiotensinogenANGT_HUMANratio: 0
A8384XANT3_HUMANAT3, SERPINC1, PRO0309Antithrombin-III precursorANT3_HUMANratio: 1000
A0647XANXA1_HUMANANXA1, ANX1, LPC1Annexin A1ANXA1_HUMANratio: 0
A0537XANXA2_HUMANANXA2, ANX2, ANX2L4Annexin A2ANXA2_HUMANratio: 0
A5831XASAH1_HUMANASAH1, ASAH, HSD33Acid ceramidase precursorASAH1_HUMANratio: 1000
A5923 BGAL_HUMANGLB1, ELNR1Beta-galactosidase precursorBGAL_HUMANratio: 0.5
A5947 BTD_HUMANBTDBiotinidase precursorBTD_HUMANratio: 1000
A5952 C1RL_HUMANC1RL, C1RL1, C1RLPComplement C1r-like proteinaseC1RL_HUMANratio: 4.75
A4614 CAD11_HUMANCDH11Cadherin-11 precursorCAD11_HUMANratio: 0
A0219 CADH2_HUMANCDH2, CDHN, NCADNeural-cadherin precursorCADH2_HUMANratio: 1000
A4628 CADM4_HUMANCADM4, IGSF4C, NECL4Cell adhesion molecule 4CADM4_HUMANratio: 2
A0008 CALM_HUMANCALM1, CALM2, CALM3CalmodulinCALM_HUMANratio: 0
A5982XCATB_HUMANCTSB, CPSB, APPSCathepsin B precursorCATB_HUMANratio: 0
A5983 CATC_HUMANCTSC, CPPIDipeptidyl-peptidase 1 precursorCATC_HUMANratio: 1000
A5984XCATD_HUMANCTSD, CPSDCathepsin D precursorCATD_HUMANratio: 1000
A8390XCBG_HUMANSERPINA6, CBGCorticosteroid-binding globulin precursorCBG_HUMANratio: 0
A6005 CBPM_HUMANCPMCarboxypeptidase M precursorCBPM_HUMANratio: 0
A6039 CEL_HUMANCEL, CELP, CELLBile salt-activated lipaseCEL_HUMANratio: 0
A6071 CK054_HUMANLP4947, PTD012Ester hydrolase C11ORF54CK054_HUMANratio: 0
A9278 CLM9_HUMANCD300LG, UNQ422, RLLV422CMRF35-like molecule 9CLM9_HUMANratio: 0
A3490 CNTFR_HUMANCNTFRCiliary neurotrophic factor receptor alpha precursorCNTFR_HUMANratio: 0
A0757 CNTN1_HUMANCNTN1Contactin-1 precursorCNTN1_HUMANratio: 1000
A1423XCO6A3_HUMANCOL6A3Collagen alpha 3(VI) chain precursorCO6A3_HUMANratio: 1000
A4711 COFA1_HUMANCOL15A1Collagen alpha 1(XV) chain precursorCOFA1_HUMANratio: 0.5
A9282 COL12_HUMANCOLEC12, CLP1, NSR2Scavenger receptor with C-type lectin type ICOL12_HUMANratio: 0
A8729 CPN2_HUMANCPN2, ACBPCarboxypeptidase N subunit 2 precursorCPN2_HUMANratio: 1000
A925BXCUBN_HUMANCUBN, IFCRCubilinCUBN_HUMANratio: 0.33
A250D CUTA_HUMANCUTA, ACHAPProtein CUTA precursorCUTA_HUMANratio: 0
A8400XCYTB_HUMANCSTB, CST6, STFBCystatin BCYTB_HUMANratio: 0
A1001 DNAS1_HUMANDNASE1, DNL1, DRNIDeoxyribonuclease 1DNAS1_HUMANratio: 0.32
A6356 DPEP1_HUMANDPEP1, MDP, RDPMicrosomal dipeptidase precursorDPEP1_HUMANratio: 0
A6367XDPP4_HUMANDPP4, ADCP2, CD26Dipeptidyl peptidase 4DPP4_HUMANratio: 0
A0609 EGF_HUMANEGFPro-epidermal growth factor precursorEGF_HUMANratio: 0.47
A6423 ENDD1_HUMANENDOD1Endonuclease domain-containing 1 protein precursorENDD1_HUMANratio: 0.3
A1636 EPHB4_HUMANEPHB4, HTK, TYRO11Ephrin type-B receptor 4 precursorEPHB4_HUMANratio: 1000
A8774 FBLN3_HUMANEFEMP1, FBLN3, FBNLEGF-containing fibulin-like extracellular matrix protein 1 precursorFBLN3_HUMANratio: 0
A8783XFETUA_HUMANAHSG, FETUA, PRO2743Alpha-2-HS-glycoprotein precursorFETUA_HUMANratio: 0
A1467XFIBA_HUMANFGAFibrinogen alpha/alpha-E chain precursorFIBA_HUMANratio: 0
A989B FRIL_HUMANFTL, FTLvariantFerritin light chainFRIL_HUMANratio: 1000
A0419XGELS_HUMANGSNGelsolin precursor, plasmaGELS_HUMANratio: 1000
A471B GOLM1_HUMANGOLPH2, GOLM1, UNQ686/PRO1326Golgi membrane protein 1GOLM1_HUMANratio: 0
A0994 GPVI_HUMANGP6, GPVIPlatelet glycoprotein VI precursorGPVI_HUMANratio: 1000
A1961XGSTP1_HUMANGSTP1, FAEES3, GST3Glutathione S-transferase PGSTP1_HUMANratio: 1000
A5356 HORN_HUMANHRNR, S100A18HornerinHORN_HUMANratio: 1000
A4856XHSPB1_HUMANHSPB1, HSP27, HSP28Heat shock protein beta-1HSPB1_HUMANratio: 0
A8243 HV312_HUMANIGH, HuVH8B VH, scFvIg heavy chain V-III regionHV306_HUMANratio: 1000
A4857XIBP7_HUMANIGFBP7, MAC25, PSFInsulin-like growth factor binding protein 7 precursorIBP7_HUMANratio: 0
A1591XIC1_HUMANSERPING1, C1IN, C1NHSerine/cysteine proteinase inhibitor clade G member 1IC1_HUMANratio: 0.43
A1411XIGHA1_HUMANIGM, SNC73, IGHA1Ig alpha-1 chain C regionIGHA1_HUMANratio: 2.41
A8363 IGHM_HUMANIGHM, IgIg mu chain CIGHM_HUMANratio: 0
A8272 IGJ_HUMANIGJ, IGCJImmunoglobulin J chainIGJ_HUMANratio: 3.92
A8844XIGSF8_HUMANIGSF8, CD81P3, EWI2Immunoglobulin superfamily member 8 precursorIGSF8_HUMANratio: 1000
A3748 K1C16_HUMANKRT16, KRT16AKeratin, type I cytoskeletal 16K1C16_HUMANratio: 1000
A3845 K22O_HUMANKRT76, KRT2B, KRT2PKeratin, type II cytoskeletal 2 oralK22O_HUMANratio: 0
A1669XK2C5_HUMANKRT5Keratin, type II cytoskeletal 5K2C5_HUMANratio: 1000
A4877XK2C6A_HUMANKRT6A, K6A, KRT6DKeratin, type II cytoskeletal 6AK2C6A_HUMANratio: 0
A3848XK2C7_HUMANKRT7, SCLKeratin, type II cytoskeletal 7K2C7_HUMANratio: 0
A6853XKLK1_HUMANKLK1, KALLIKREINKallikrein 1KLK1_HUMANratio: 0
A6859 KLK3_HUMANKLK3, APSProstate specific antigen precursorKLK3_HUMANratio: 1000
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainKV201_HUMANratio: 0.41
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainKV302_HUMANratio: 1000
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainKV310_HUMANratio: 1000
A8364XLV605_HUMANIGL, IGLV3-22, V2-15Ig lambda chainLAC2_HUMANratio: 1000
A8364XLV605_HUMANIGL, IGLV3-22, V2-15Ig lambda chainLAC6_HUMANratio: 0
A1010 LAIR1_HUMANLAIR1, CD305Leukocyte-associated IG-like receptor 1 precursorLAIR1_HUMANratio: 0.21
A1632XLCAT_HUMANLCATPhosphatidylcholine-sterol acyltransferase precursorLCAT_HUMANratio: 1000
A4951XLUM_HUMANLUM, LDC, SLRR2DLumican precursorLUM_HUMANratio: 2.08
A8364XLV605_HUMANIGL, IGLV3-22, V2-15Ig lambda chainLV403_HUMANratio: 1000
A0808XMOES_HUMANMSNMoesinMOES_HUMANratio: 1000
A4986 MUC1_HUMANMUC1, PUMMucin-1MUC1_HUMANratio: 0.36
A224C NGAL_HUMANLCN2, HNL, NGALNeutrophil gelatinase-associated lipocalin precursorNGAL_HUMANratio: 0
A1585XNID1_HUMANNID1, NIDNidogen-1NID1_HUMANratio: 0.43
A9427 OSCAR_HUMANOSCAR, PIGR3Osteoclast-associated immunoglobulin-like receptorOSCAR_HUMANratio: 0
A8928 PEBP4_HUMANPEBP4, CORK1, UNQ1933/PRO4408Phosphatidylethanolamine-binding protein 4PEBP4_HUMANratio: 0
BC149 PEPA3_HUMANPGA3, PGA4Pepsin A-3PEPA3_HUMANratio: 0
BC149 PEPA3_HUMANPGA3, PGA4Pepsin A-3PEPA3_HUMANratio: 1000
A7471 PPAL_HUMANACP2Lysosomal acid phosphatase precursorPPAL_HUMANratio: 1000
A7472 PPAP_HUMANACPPProstatic acid phosphatase precursorPPAP_HUMANratio: 1000
A1544 PROZ_HUMANPROZVitamin K-dependent protein Z precursorPROZ_HUMANratio: 1000
A7540XPTGDS_HUMANPTGDS, PDS, PGDS2Prostaglandin-H2 D-isomerase precursorPTGDS_HUMANratio: 2.83
A5448 REG1A_HUMANREG1A, PSPS, PSPS1Lithostathine 1 alpha precursorREG1A_HUMANratio: 1000
A353CXRET4_HUMANRBP4, PRO2222, PRBPRetinol binding protein 4, plasmaRET4_HUMANratio: 0
A1255 RL40_HUMANUBA52, UBCEP2, CEP52Ubiquitin and ribosomal protein L40 precursorRL40_HUMANratio: 1000
A7710 RNAS2_HUMANRNASE2, EDN, RNS2Nonsecretory ribonuclease precursorRNAS2_HUMANratio: 0.26
A1550 S10A8_HUMANS100A8, CAGA, CFAGCalgranulin AS10A8_HUMANratio: 0
A1551 S10A9_HUMANS100A9, CAGB, CFAGProtein S100-A9S10A9_HUMANratio: 0
A0519 SHPS1_HUMANSIRPA, BIT, MFRProtein-tyrosine phosphatase non-receptor type substrate 1 precursorSHPS1_HUMANratio: 0
A9055 SIRBL_HUMANSIRPB1Signal-regulatory protein beta-1SIRB1_HUMANratio: 0
A9055 SIRBL_HUMANSIRPB1Signal-regulatory protein beta-1SIRBL_HUMANratio: 1000
A7971 TGM4_HUMANTGM4, hTGP, HTGPProtein-glutamine glutamyltransferase 4TGM4_HUMANratio: 1000
A8016XTPP1_HUMANTPP1, CLN2, GIG1Tripeptidyl-peptidase I precursorTPP1_HUMANratio: 0
A643CXTRFE_HUMANTF, PRO1400Serotransferrin precursorTRFE_HUMANratio: 3.3
A648CXTTHY_HUMANTTR, PALB, TBPATransthyretin precursorTTHY_HUMANratio: 1000
A9139 UROM_HUMANUMODUromodulin precursorUROM_HUMANratio: 0.49
A9149XVTDB_HUMANGCVitamin D-binding protein precursorVTDB_HUMANratio: 0.44
A1471XVTNC_HUMANVTNVitronectin precursorVTNC_HUMANratio: 0
A722C ZG16B_HUMANZG16B, UNQ773/PRO1567, EECPZymogen granule protein 16 homolog BZG16B_HUMANratio: 0

Compile date 08-10-2018© iMODE-CKD consortium