CKDdb-logoCKDdb - Study ID Exp23344851

PubMed ID23344851
AuthorsRaimondo F, Corbetta S, Morosi L, Chinello C, Gianazza E, Castoldi G, Di Gioia C, Bombardi C, Stella A, Battaglia C, Bianchi C, Magni F, Pitto M.
TitleUrinary exosomes and diabetic nephropathy: a proteomic approach.
JournalMol Biosyst. 2013 Jun;9(6):1139-46.
AbstractUrinary exosomes (UE) are nanovesicles released by every epithelial cell facing the urinary space and they are considered a promising source of molecular markers for renal dysfunction and structural injury. Exosomal proteomics has emerged as a powerful tool for understanding the molecular composition of exosomes and has potential to accelerate biomarker discovery. We employed this strategy in the study of diabetic nephropathy (DN) and the consequent end stage renal disease, which represent the dramatic evolution of diabetes, often leading the patients to dialysis or kidney transplantation. The identification of DN biomarkers is likely to help monitoring the disease onset and progression. A label free LC-MS/MS approach was applied to investigate the alteration of the proteome of urinary exosomes isolated from the Zucker diabetic fatty rats (ZDF), as a model of type 2 DN. We collected 24 hour urine samples from 7 ZDF and from 7 control rats at different ages (6, 12 and 20 weeks old) to monitor the development of DN. Exosomes were isolated by ultracentrifugation and their purity assessed by immunoblotting for known exosomal markers. Exosomal proteins from urine samples of 20 week old rats were pooled and analyzed by nLC-ESI-UHR-QToF-MS/MS after pre-filtration and tryptic digestion, leading to the identification and label free quantification of 286 proteins. Subcellular localization and molecular functions were assigned to each protein by UniprotKB, showing that the majority of identified proteins were membrane-associated or cytoplasmic and involved in transport, signalling and cellular adhesion, typical functions of exosomal proteins. We further validated label free mass spectrometry results by immunoblotting, as exemplified by: Xaa-Pro dipeptidase, Major Urinary Protein 1 and Neprilysin, which resulted increased, decreased and not different, respectively, in exosomes isolated from diabetic urine samples compared to controls, by both techniques. In conclusion we show the potential of exosome proteomics for DN biomarker discovery.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Rattus norvegicusurineexosomeDiabetes14nLC-ESI-UHR-QToF-MS/MSDem23344851

Sample description and preparation

N (case)7
N (control)7
Disease (case)diabetic nephropathy
Disease (control)healthy
Disease inductionZucker diabetic fatty rats, mutation in the gene coding the leptin receptor (fa/fa)
Digestin-solution, trypsin

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)Scores
A1961XGSTP1_RATGstp1Glutathione S-transferase PGSTP1_RATquantification method: spectral counting
A5189 TBB2A_RATTubb2aTubulin beta-2A chainTBB2A_RATquantification method: spectral counting
A5632 AADAT_RATAadat, Kat2Kynurenine/alpha-aminoadipate aminotransferaseAADAT_RATquantification method: spectral counting
A7842 SODC_RATSod1Superoxide dismutase [Cu-Zn]SODC_RATquantification method: spectral counting
A7902 SUIS_RATSiSucrase-isomaltaseSUIS_RATquantification method: spectral counting
A8037 TRM11_RATTrmt11tRNA (guanine(10)-N2)-methyltransferase homologTRM11_RATquantification method: spectral counting
A8533 ZPI_RATSerpina10, Rasp1, ZpiProtein Z-dependent protease inhibitorZPI_RATquantification method: spectral counting
A0280 1433E_RATYwhae14-3-3 protein epsilon1433E_RATquantification method: spectral counting
A0361 1433Z_RATYwhaz, Msfs114-3-3 protein zeta/delta1433Z_RATquantification method: spectral counting
A8368 22P2_RATCrp2, P22k15Cystatin-related protein 222P2_RATquantification method: spectral counting
A5615X3HAO_RATHaao3-hydroxyanthranilate 33HAO_RATquantification method: spectral counting
A8369XA1AT_RATSerpina1Alpha-1-antiproteinaseA1AT_RATquantification method: spectral counting
A8376 A1I3_RATA1i3, Mug1Alpha-1-inhibitor 3A1I3_RATquantification method: spectral counting
A8377 A1M_RATA1m, PzpAlpha-1-macroglobulinA1M_RATquantification method: spectral counting
A784B ABCG2_RATAbcg2, Bcrp1, Abcg3l2ATP-binding cassette sub-family G member 2ABCG2_RATquantification method: spectral counting
A5647XABHEB_RATAbhd14bAlpha/beta hydrolase domain-containing protein 14BABHEB_RATquantification method: spectral counting
A5670 ACOC_RATAco1, Ireb1, IrebpCytoplasmic aconitate hydrataseACOC_RATquantification method: spectral counting
A4548 ACTB_RATActbActinACTB_RATquantification method: spectral counting
A4551 ACTC_RATActc1, ActcActinACTC_RATquantification method: spectral counting
A2341XACTN1_RATActn1Alpha-actinin-1ACTN1_RATquantification method: spectral counting
A5703 ACY1A_RATACY1B_RATAcy1a, Acy1, Acy1bAminoacylase-1AACY1A_RATquantification method: spectral counting
A5704XACY2_RATAspaAspartoacylaseACY2_RATquantification method: spectral counting
A5705 ACY3_RATAcy3N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)ACY3_RATquantification method: spectral counting
A5750 AK1A1_RATAkr1a1, AlrAlcohol dehydrogenase [NADP(+)]AK1A1_RATquantification method: spectral counting
A5766 AL4A1_RATAldh4a1, LOC641316, Tas1r2Delta-1-pyrroline-5-carboxylate dehydrogenaseAL4A1_RATquantification method: spectral counting
A5769 AL9A1_RATAldh9a14-trimethylaminobutyraldehyde dehydrogenaseAL9A1_RATquantification method: spectral counting
A1581XALBU_RATAlbSerum albumin ( Precursor)ALBU_RATquantification method: spectral counting
A1924 ALDOB_RATAldobFructose-bisphosphate aldolase BALDOB_RATquantification method: spectral counting
A8383XAMBP_RATAmbp, ItilProtein AMBPAMBP_RATquantification method: spectral counting
A5796 AMPE_RATEnpepGlutamyl aminopeptidaseAMPE_RATquantification method: spectral counting
A5797 AMPL_RATLap3Cytosol aminopeptidaseAMPL_RATquantification method: spectral counting
A5800 AMPN_RATAnpep, APNAminopeptidase NAMPN_RATquantification method: spectral counting
A5805XAMYP_RATAmy2, Amy2a3, Amy2-3Pancreatic alpha-amylaseAMYP_RATquantification method: spectral counting
A1702XANGT_RATAgt, Serpina8AngiotensinogenANGT_RATquantification method: spectral counting
A8385 ANXA3_RATAnxa3, Anx3Annexin A3ANXA3_RATquantification method: spectral counting
A4575XANXA4_RATAnxa4, Anx4Annexin A4ANXA4_RATquantification method: spectral counting
A4576 ANXA5_RATAnxa5, Anx5Annexin A5ANXA5_RATquantification method: spectral counting
A0029XANXA6_RATAnxa6, Anx6Annexin A6ANXA6_RATquantification method: spectral counting
A1552 APOA1_RATApoa1Apolipoprotein A-IAPOA1_RATquantification method: spectral counting
A810BXAPOA4_RATApoa4Apolipoprotein A-IVAPOA4_RATquantification method: spectral counting
A812BXAPOC1_RATApoc1Apolipoprotein C-IAPOC1_RATquantification method: spectral counting
A1176 APOE_RATApoeApolipoprotein EAPOE_RATquantification method: spectral counting
A824B AQP1_RATAqp1, Chip28Aquaporin-1AQP1_RATquantification method: spectral counting
A0455 ARF1_RATArf1ADP-ribosylation factor 1ARF1_RATquantification method: spectral counting
A832B ARF4_RATArf4ADP-ribosylation factor 4ARF4_RATquantification method: spectral counting
A5824 ARK72_RATAkr7a2, Afar2, AiarAflatoxin B1 aldehyde reductase member 2ARK72_RATquantification method: spectral counting
A5825 ARK73_RATAkr7a3, Afar, Akr7a1Aflatoxin B1 aldehyde reductase member 3ARK73_RATquantification method: spectral counting
A0340 AT1A1_RATAtp1a1Sodium/potassium-transporting ATPase subunit alpha-1AT1A1_RATquantification method: spectral counting
A0344 AT1B1_RATAtp1b1Sodium/potassium-transporting ATPase subunit beta-1AT1B1_RATquantification method: spectral counting
A434F ATGA1_RATAtg101Autophagy-related protein 101ATGA1_RATquantification method: spectral counting
A3574XBASP1_RATBasp1, Nap22Brain acid soluble protein 1BASP1_RATquantification method: spectral counting
A5928XBIEA_RATBlvra, BlvrBiliverdin reductase ABIEA_RATquantification method: spectral counting
A869C BROX_RATBroxBRO1 domain-containing protein BROXBROX_RATquantification method: spectral counting
A5964XCAH2_RATCa2Carbonic anhydrase 2CAH2_RATquantification method: spectral counting
A704F CD051_RAT Uncharacterized protein C4orf51 homologCD051_RATquantification method: spectral counting
A1093 CD47_RATCd47Leukocyte surface antigen CD47CD47_RATquantification method: spectral counting
A1630 CD59_RATCd59CD59 glycoproteinCD59_RATquantification method: spectral counting
A5528 CD63_RATCd63CD63 antigenCD63_RATquantification method: spectral counting
A4646 CD9_RATCd9CD9 antigenCD9_RATquantification method: spectral counting
A0155 CDC42_RATCdc42Cell division control protein 42 homolog ( Precursor)CDC42_RATquantification method: spectral counting
A4651XCDHR5_RATCdhr5, Mucdhl, MupcdhCadherin-related family member 5CDHR5_RATquantification method: spectral counting
A6042 CERU_RATCp, CERPCeruloplasminCERU_RATquantification method: spectral counting
A102F CES1D_RATCes1d, Ces3Carboxylesterase 1DCES3_RATquantification method: spectral counting
A6045 CGL_RATCth, CSECystathionine gamma-lyaseCGL_RATquantification method: spectral counting
A6071 CK054_RAT Ester hydrolase C11orf54 homologCK054_RATquantification method: spectral counting
A0119XCLH1_RATCltcClathrin heavy chain 1CLH_RATquantification method: spectral counting
A4443 CLIC1_RATClic1Chloride intracellular channel protein 1CLIC1_RATquantification method: spectral counting
A4446XCLIC4_RATClic4Chloride intracellular channel protein 4CLIC4_RATquantification method: spectral counting
A885BXCLPB_RATClpb, Skd3Caseinolytic peptidase B protein homologCLPB_RATquantification method: spectral counting
A1276XCLUS_RATCluClusterinCLUS_RATquantification method: spectral counting
A6086 CNDP1_RATCndp1, Cn1Beta-Ala-His dipeptidaseCNDP1_RATquantification method: spectral counting
A6087XCNDP2_RATCndp2Cytosolic non-specific dipeptidaseCNDP2_RATquantification method: spectral counting
A1598XCO3_RATC3Complement C3CO3_RATquantification method: spectral counting
A8720XCO4_RATC4, C4a, ApomComplement C4CO4_RATquantification method: spectral counting
A1628 CO9_RATC9Complement component C9 ( Precursor)CO9_RATquantification method: spectral counting
A0999 COF1_RATCfl1Cofilin-1COF1_RATquantification method: spectral counting
A6092 COMT_RATComtCatechol O-methyltransferaseCOMT_RATquantification method: spectral counting
A4725 COTL1_RATCotl1, ClpCoactosin-like proteinCOTL1_RATquantification method: spectral counting
A915BXCPNE9_RATCpne9Copine-9CPNE9_RATquantification method: spectral counting
A6204 CPT1B_RATCpt1b, CPTICarnitine O-palmitoyltransferase 1CPT1B_RATquantification method: spectral counting
A6212 CRP_RATCrp, Ptx1C-reactive protein ( Precursor)CRP_RATquantification method: spectral counting
A6213 CRYL1_RATCryl1, CryLambda-crystallin homologCRYL1_RATquantification method: spectral counting
A8735 CRYM_RATCrymKetimine reductase mu-crystallinCRYM_RATquantification method: spectral counting
A414B CTL4_RATSlc44a4, Ctl4, Ng22Choline transporter-like protein 4CTL4_RATquantification method: spectral counting
A0461 DDAH1_RATDdah1, DdahN(G)DDAH1_RATquantification method: spectral counting
A4750 DEST_RATDstn, LOC680058DestrinDEST_RATquantification method: spectral counting
A6306 DHAK_RATDakBifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)DHAK_RATquantification method: spectral counting
A6314 DHPR_RATQdpr, DhprDihydropteridine reductaseDHPR_RATquantification method: spectral counting
A6321 DHSO_RATSord, Sdh1Sorbitol dehydrogenaseDHSO_RATquantification method: spectral counting
A1001 DNAS1_RATDnase1, Dnl1Deoxyribonuclease-1DNAS1_RATquantification method: spectral counting
A6354 DOPD_RATDdtD-dopachrome decarboxylaseDOPD_RATquantification method: spectral counting
A6365XDPP2_RATDpp7, Dpp2Dipeptidyl peptidase 2DPP2_RATquantification method: spectral counting
A6367XDPP4_RATDpp4, Cd26Dipeptidyl peptidase 4DPP4_RATquantification method: spectral counting
A1820 EF1A1_RATEef1a1, Eef1a, LOC100360150Elongation factor 1-alpha 1EF1A1_RATquantification method: spectral counting
A3804 EF2_RATEef2, Dapk3, Ef-2Elongation factor 2EF2_RATquantification method: spectral counting
A0609 EGF_RATEgfPro-epidermal growth factorEGF_RATquantification method: spectral counting
A1319XEHD1_RATEhd1EH domain-containing protein 1EHD1_RATquantification method: spectral counting
A3615 ENOA_RATEno1, Eno-1Alpha-enolaseENOA_RATquantification method: spectral counting
A2468 ENPP3_RATEnpp3, Pdnp3, pdnpnoEctonucleotide pyrophosphatase/phosphodiesterase family member 3ENPP3_RATquantification method: spectral counting
A2472 ENPP6_RATEnpp6, Enpp6_predictedEctonucleotide pyrophosphatase/phosphodiesterase family member 6ENPP6_RATquantification method: spectral counting
A6464 ESTD_RATEsdS-formylglutathione hydrolaseESTD_RATquantification method: spectral counting
A0326 EZRI_RATEzr, Vil2EzrinEZRI_RATquantification method: spectral counting
A431BXF151A_RATFam151aProtein FAM151AF151A_RATquantification method: spectral counting
A6477 F16P1_RATFbp1, FbpFructose-1F16P1_RATquantification method: spectral counting
A6483 FAAA_RATFahFumarylacetoacetaseFAAA_RATquantification method: spectral counting
A8783XFETUA_RATAhsg, FetuaAlpha-2-HS-glycoproteinFETUA_RATquantification method: spectral counting
A462D FETUB_RATFetubFetuin-BFETUB_RATquantification method: spectral counting
A6510 FIGL1_RATFignl1, S30Fidgetin-like protein 1FIGL1_RATquantification method: spectral counting
A1466XFINC_RATFn1, fn-1FibronectinFINC_RATquantification method: spectral counting
A6524XFRIH_RATFth1, FthFerritin heavy chainFRIH_RATquantification method: spectral counting
A165F FRIL1_RATFtl1, FtlFerritin light chain 1FRIL1_RATquantification method: spectral counting
A0331 G3P_RATGapdh, GapdGlyceraldehyde-3-phosphate dehydrogenaseG3P_RATquantification method: spectral counting
A6543XG3PT_RATGapdhs, Gapd-s, GapdsGlyceraldehyde-3-phosphate dehydrogenaseG3PT_RATquantification method: spectral counting
A6551 G6PI_RATGpiGlucose-6-phosphate isomeraseG6PI_RATquantification method: spectral counting
A2000 GABR2_RATGabbr2, Gpr51, GABABR2Gamma-aminobutyric acid type B receptor subunit 2GABR2_RATquantification method: spectral counting
A0069 GBB1_RATGnb1Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1GBB1_RATquantification method: spectral counting
A0070 GBB2_RATGnb2Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2GBB2_RATquantification method: spectral counting
A0071 GBB3_RATGnb3Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3GBB3_RATquantification method: spectral counting
A8804 GDIB_RATGdi2, Gdi3Rab GDP dissociation inhibitor betaGDIB_RATquantification method: spectral counting
A0704 GDIR1_RATArhgdiaRho GDP-dissociation inhibitor 1GDIR1_RATquantification method: spectral counting
A0496 GGT1_RATGgt1, GgtGamma-glutamyltranspeptidase 1GGT1_RATquantification method: spectral counting
A1159 GIPC1_RATGipc1, Gipc, Rgs19ip1PDZ domain-containing protein GIPC1GIPC1_RATquantification method: spectral counting
A1723XGIPC2_RATGipc2PDZ domain-containing protein GIPC2GIPC2_RATquantification method: spectral counting
A0424 GLNA_RATGlul, GlnsGlutamine synthetaseGLNA_RATquantification method: spectral counting
A0044 GNA11_RATGna11Guanine nucleotide-binding protein subunit alpha-11GNA11_RATquantification method: spectral counting
A0054 GNAI1_RATGnai1, Gnai-1Guanine nucleotide-binding protein G(i) subunit alpha-1GNAI1_RATquantification method: spectral counting
A0050 GNAI3_RATGnai3, Gnai-3Guanine nucleotide-binding protein G(k) subunit alphaGNAI3_RATquantification method: spectral counting
A8814 GNAS1_RATGnas, Gnas1, XLasGuanine nucleotide-binding protein G(s) subunit alpha isoforms XLasGNAS1_RATquantification method: spectral counting
A505D GP2_RATGp2Pancreatic secretory granule membrane major glycoprotein GP2GP2_RATquantification method: spectral counting
A389J GPX41_RATGpx4Phospholipid hydroperoxide glutathione peroxidaseGPX41_RATquantification method: spectral counting
A0301 GRIP1_RATGrip1Glutamate receptor-interacting protein 1GRIP1_RATquantification method: spectral counting
A4128XGSH0_RATGclm, Glclr, GclnGlutamate--cysteine ligase regulatory subunitGSH0_RATquantification method: spectral counting
A6662 GSH1_RATGclc, GlclcGlutamate--cysteine ligase catalytic subunitGSH1_RATquantification method: spectral counting
A6663 GSHB_RATGssGlutathione synthetaseGSHB_RATquantification method: spectral counting
A1949 GSTA1_RATGsta1, Gsta3, GSTGlutathione S-transferase alpha-1GSTA1_RATquantification method: spectral counting
A1950XGSTA3_RATGsta3, Gstyc1Glutathione S-transferase alpha-3GSTA3_RATquantification method: spectral counting
A1955 GSTM2_RATGstm2Glutathione S-transferase Mu 2GSTM2_RATquantification method: spectral counting
A1969 GSTO1_RATGsto1Glutathione S-transferase omega-1GSTO1_RATquantification method: spectral counting
A6670XGSTT2_RATGstt2Glutathione S-transferase theta-2GSTT2_RATquantification method: spectral counting
A013C GTR5_RATSlc2a5, Glut5Solute carrier family 2GTR5_RATquantification method: spectral counting
A1028 H2A1C_RAT Histone H2A type 1-CH2A1C_RATquantification method: spectral counting
A1060 H4_RATHist1h4b, Hist1h4m, Hist4h4Histone H4H4_RATquantification method: spectral counting
A1741 HBA_RATHba1, Hba-a1, LOC287167Hemoglobin subunit alpha-1/2HBA_RATquantification method: spectral counting
A016C HBB1_RATHbbHemoglobin subunit beta-1HBB1_RATquantification method: spectral counting
A017C HBB2_RAT Hemoglobin subunit beta-2HBB2_RATquantification method: spectral counting
A021CXHEMO_RATHpxHemopexin ( Precursor)HEMO_RATquantification method: spectral counting
A8414 HEP2_RATSerpind1, Hcf2Heparin cofactor 2HEP2_RATquantification method: spectral counting
A1481 HINT1_RATHint1, Hint, Pkci1Histidine triad nucleotide-binding protein 1HINT1_RATquantification method: spectral counting
A6737 HNMT_RATHnmtHistamine N-methyltransferaseHNMT_RATquantification method: spectral counting
A8835 HPCL1_RATHpcal1Hippocalcin-like protein 1HPCL1_RATquantification method: spectral counting
A1631XHPT_RATHpHaptoglobinHPT_RATquantification method: spectral counting
A1611XHRG_RATHrg, Hrg1Histidine-rich glycoproteinHRG_RATquantification method: spectral counting
A0191 HS90A_RATHsp90aa1, Hsp86, HspcaHeat shock protein HSP 90-alphaHS90A_RATquantification method: spectral counting
A0146 HSP7C_RATHspa8, Hsc70, Hsc73Heat shock cognate 71 kDa proteinHSP7C_RATquantification method: spectral counting
A6772 IAH1_RATIah1, Harpb64Isoamyl acetate-hydrolyzing esterase 1 homologIAH1_RATquantification method: spectral counting
A3661XIDHC_RATIdh1Isocitrate dehydrogenase [NADP] cytoplasmicIDHC_RATquantification method: spectral counting
A8266 IGG2A_RATIgg-2aIg gamma-2A chain C regionIGG2A_RATquantification method: spectral counting
A8267 IGG2B_RATIgh-1aIg gamma-2B chain C regionIGG2B_RATquantification method: spectral counting
A8270XIGG2C_RATIghg3, Ighv13-1Ig gamma-2C chain C regionIGG2C_RATquantification method: spectral counting
A4136 IST1_RATIst1IST1 homologIST1_RATquantification method: spectral counting
A8436 ITM2B_RATItm2bIntegral membrane protein 2BITM2B_RATquantification method: spectral counting
A3824 K1C10_RATKrt10, Ka10KeratinK1C10_RATquantification method: spectral counting
A3785 K1C15_RATKrt15, Ka15KeratinK1C15_RATquantification method: spectral counting
A3849 K2C1_RATKrt1, Kb1KeratinK2C1_RATquantification method: spectral counting
A8277 KACB_RAT Ig kappa chain C regionKACB_RATquantification method: spectral counting
A4475 KCMB2_RATKcnmb2Calcium-activated potassium channel subunit beta-2KCMB2_RATquantification method: spectral counting
A6849 KHK_RATKhkKetohexokinaseKHK_RATquantification method: spectral counting
A070C KIF5A_RATKif5aKinesin heavy chain isoform 5AKIF5A_RATquantification method: spectral counting
A9969 KNT1_RATMap1T-kininogen 1KNT1_RATquantification method: spectral counting
A9970 KNT2_RATKng1l1, Kng1T-kininogen 2KNT2_RATquantification method: spectral counting
A406J LAC2_RATLOC100361706Ig lambda-2 chain C regionLAC2_RATquantification method: spectral counting
A1327 LAMP1_RATLamp1, Lamp-1Lysosome-associated membrane glycoprotein 1LAMP1_RATquantification method: spectral counting
A3882 LASP1_RATLasp1LIM and SH3 domain protein 1LASP1_RATquantification method: spectral counting
A1610 LRP2_RATLrp2Low-density lipoprotein receptor-related protein 2LRP2_RATquantification method: spectral counting
A6982 MCES_RATRnmtmRNA cap guanine-N7 methyltransferaseMCES_RATquantification method: spectral counting
A6992 MDHC_RATMdh1, MdhMalate dehydrogenaseMDHC_RATquantification method: spectral counting
A6997 MEP1A_RATMep1aMeprin A subunit alphaMEP1A_RATquantification method: spectral counting
A6998 MEP1B_RATMep1bMeprin A subunit betaMEP1B_RATquantification method: spectral counting
A7019 MGLL_RATMgll, Mgl2Monoglyceride lipaseMGLL_RATquantification method: spectral counting
A7027 MIF_RATMif, LOC686548, LOC679748Macrophage migration inhibitory factorMIF_RATquantification method: spectral counting
A354H MIPT3_RATTraf3ip1, Ift54, Mipt3TRAF3-interacting protein 1MIPT3_RATquantification method: spectral counting
A0265 MK03_RATMapk3, Erk1, Prkm3Mitogen-activated protein kinase 3MK03_RATquantification method: spectral counting
A364H MKS3_RATTmem67, Mks3, WpkMeckelinMKS3_RATquantification method: spectral counting
A0808XMOES_RATMsnMoesinMOES_RATquantification method: spectral counting
A149C MUP_RATLC11, LOC259246Major urinary proteinMUP_RATquantification method: spectral counting
A4049 MYL6_RATMyl6Myosin light polypeptide 6MYL6_RATquantification method: spectral counting
A172C MYO1C_RATMyo1c, Myr2Unconventional myosin-IcMYO1C_RATquantification method: spectral counting
A3959 MYO1D_RATMyo1d, Myr4Unconventional myosin-IdMYO1D_RATquantification method: spectral counting
A493H NAR5_RATArt5Ecto-ADP-ribosyltransferase 5NAR5_RATquantification method: spectral counting
A7149 NEP_RATMmeNeprilysinNEP_RATquantification method: spectral counting
A0806 NHRF1_RATSlc9a3r1, Nherf, Nherf1Na(+)/H(+) exchange regulatory cofactor NHE-RF1NHRF1_RATquantification method: spectral counting
A9732 NHRF3_RATPdzk1, Clamp, Diphor1Na(+)/H(+) exchange regulatory cofactor NHE-RF3NHRF3_RATquantification method: spectral counting
A7158 NIT1_RATNit1Nitrilase homolog 1NIT1_RATquantification method: spectral counting
A234CXNPT2A_RATSlc34a1, Slc17a2, NaPi-2Sodium-dependent phosphate transport protein 2ANPT2A_RATquantification method: spectral counting
A608H NR4A3_RATNr4a3, Nor1Nuclear receptor subfamily 4 group A member 3NR4A3_RATquantification method: spectral counting
A257C NUP93_RATNup93Nuclear pore complex protein Nup93NUP93_RATquantification method: spectral counting
A790D PBAS_RATPbsn, PrbsProbasinPBAS_RATquantification method: spectral counting
A277CXPDC6I_RATPdcd6ip, Aip1Programmed cell death 6-interacting proteinPDC6I_RATquantification method: spectral counting
A4361 PDILT_RATPdiltProtein disulfide-isomerase-like protein of the testis ( Precursor)PDILT_RATquantification method: spectral counting
A1328 PEBP1_RATPebp1, Pbp, PebpPhosphatidylethanolamine-binding protein 1PEBP1_RATquantification method: spectral counting
A796D PEF1_RATPef1PeflinPEF1_RATquantification method: spectral counting
A7357XPEPD_RATPepdXaa-Pro dipeptidasePEPD_RATquantification method: spectral counting
A7363 PGAM1_RATPgam1Phosphoglycerate mutase 1PGAM1_RATquantification method: spectral counting
A7372 PGK1_RATPgk1, Pgk-1, RGD1560402Phosphoglycerate kinase 1PGK1_RATquantification method: spectral counting
A0919 PLEK_RATPlekPleckstrinPLEK_RATquantification method: spectral counting
A1468XPLMN_RATPlgPlasminogenPLMN_RATquantification method: spectral counting
A5087XPLST_RATPls3Plastin-3PLST_RATquantification method: spectral counting
A5088XPODXL_RATPodxl, Pclp1PodocalyxinPODXL_RATquantification method: spectral counting
A7471 PPAL_RATAcp2Lysosomal acid phosphatasePPAL_RATquantification method: spectral counting
A7476 PPBT_RATAlplAlkaline phosphatasePPBT_RATquantification method: spectral counting
A4369 PPIA_RATPpiaPeptidyl-prolyl cis-trans isomerase APPIA_RATquantification method: spectral counting
A0371 PRDX1_RATPrdx1, Tdpx2Peroxiredoxin-1PRDX1_RATquantification method: spectral counting
A7503XPRDX5_RATPrdx5Peroxiredoxin-5PRDX5_RATquantification method: spectral counting
A0432 PRDX6_RATPrdx6, Aipla2, Aop2Peroxiredoxin-6PRDX6_RATquantification method: spectral counting
A0524 PROF1_RATPfn1Profilin-1PROF1_RATquantification method: spectral counting
A7539 PTER_RATPter, Rpr-1Phosphotriesterase-related proteinPTER_RATquantification method: spectral counting
A292J PTHY_RATPth, PTH-(1-84)Parathyroid hormonePTHY_RATquantification method: spectral counting
A296C PTTG_RATPttg1ipPituitary tumor-transforming gene 1 protein-interacting proteinPTTG_RATquantification method: spectral counting
A7646 QOR_RATCryzQuinone oxidoreductaseQOR_RATquantification method: spectral counting
A332CXRAB7A_RATRab7a, Rab7Ras-related protein Rab-7aRAB7A_RATquantification method: spectral counting
A0620 RAB8A_RATRab8a, Rab8Ras-related protein Rab-8A ( Precursor)RAB8A_RATquantification method: spectral counting
A0194 RAC1_RATRNS1D_RATRac1, Rnase1d, Rnase1Ras-related C3 botulinum toxin substrate 1RAC1_RATquantification method: spectral counting
A0323 RAP1A_RATRap1a, Krev1Ras-related protein Rap-1ARAP1A_RATquantification method: spectral counting
A0619 RB11A_RATRab11a, Rab11Ras-related protein Rab-11ARB11A_RATquantification method: spectral counting
A7681 RGN_RATRgn, Smp30RegucalcinRGN_RATquantification method: spectral counting
A9019 RHOA_RATRhoa, Arha, Arha2Transforming protein RhoA ( Precursor)RHOA_RATquantification method: spectral counting
A0036 RIMB1_RATBzrap1, Rbp1Peripheral-type benzodiazepine receptor-associated protein 1RIMB1_RATquantification method: spectral counting
A9646 RS27A_RATRps27a, Uba80, Ubcep1Ubiquitin-40S ribosomal protein S27aRS27A_RATquantification method: spectral counting
A0536 S10A6_RATS100a6, CacyProtein S100-A6S10A6_RATquantification method: spectral counting
A376C S12A3_RATSlc12a3, TscSolute carrier family 12 member 3S12A3_RATquantification method: spectral counting
A385CXS15A2_RATSlc15a2, Pept2Solute carrier family 15 member 2S15A2_RATquantification method: spectral counting
S1C2A_RAT S1C2A_RATSult1c2aSulfotransferase 1C2AS1C2A_RATquantification method: spectral counting
A392C S22A4_RATSlc22a4, Octn1Solute carrier family 22 member 4S22A4_RATquantification method: spectral counting
A406C S23A1_RATSlc23a1, Svct1Solute carrier family 23 member 1S23A1_RATquantification method: spectral counting
A420C S28A1_RATSlc28a1, Cnt1Sodium/nucleoside cotransporter 1S28A1_RATquantification method: spectral counting
A300IXS47A1_RATSlc47a1, Mate1Multidrug and toxin extrusion protein 1S47A1_RATquantification method: spectral counting
A304IXS6A18_RATSlc6a18, Xtrp2Sodium-dependent neutral amino acid transporter B(0)AT3S6A18_RATquantification method: spectral counting
A294J S6A20_RATSlc6a20, Sit1, Xtrp3Sodium- and chloride-dependent transporter XTRP3S6A20_RATquantification method: spectral counting
A305I S7A13_RATSlc7a13Solute carrier family 7 member 13S7A13_RATquantification method: spectral counting
A1597 SAMP_RATApcs, Ptx2, SapSerum amyloid P-componentSAMP_RATquantification method: spectral counting
A4212XSAP_RATPsap, Sgp1Sulfated glycoprotein 1SAP_RATquantification method: spectral counting
A463C SBP1_RATSelenbp1, SbpSelenium-binding protein 1SBP1_RATquantification method: spectral counting
A474CXSC5A1_RATSlc5a1, Sglt1Sodium/glucose cotransporter 1SC5A1_RATquantification method: spectral counting
A475C SC5A2_RATSlc5a2, Sglt2, sglt2deltaSodium/glucose cotransporter 2SC5A2_RATquantification method: spectral counting
A337I SC5A6_RATSlc5a6, SmvtSodium-dependent multivitamin transporterSC5A6_RATquantification method: spectral counting
A480C SC5AB_RATSlc5a11, Kst1, Smit2Sodium/myo-inositol cotransporter 2SC5AB_RATquantification method: spectral counting
A0497 SDCB1_RATSdcbpSyntenin-1SDCB1_RATquantification method: spectral counting
A307E SG2A2_RATScgb2a2, Psbpc3Secretoglobin family 2A member 2SG2A2_RATquantification method: spectral counting
A508C SLC31_RATSlc3a1, NbatNeutral and basic amino acid transport protein rBATSLC31_RATquantification method: spectral counting
SMR1_RAT SMR1_RATVcsa1SMR1 proteinSMR1_RATquantification method: spectral counting
A530C SO1A1_RATSlco1a1, Oatp1, Oatp1a1Solute carrier organic anion transporter family member 1A1SO1A1_RATquantification method: spectral counting
SO1A3_RAT SO1A3_RATSlco1a3Solute carrier organic anion transporter family member 1A3SO1A3_RATquantification method: spectral counting
A8492 SPA3K_RATSerpina3k, Spin2bSerine protease inhibitor A3KSPA3K_RATquantification method: spectral counting
A0353 SPA3L_RATSerpina3l, Spin2aSerine protease inhibitor A3LSPA3L_RATquantification method: spectral counting
A8493XSPA3M_RATSerpina3mSerine protease inhibitor A3MSPA3M_RATquantification method: spectral counting
A8494 SPA3N_RATSerpina3n, Spin2cSerine protease inhibitor A3NSPA3N_RATquantification method: spectral counting
A337E SPBP_RATSbpProstatic spermine-binding proteinSPBP_RATquantification method: spectral counting
A7879 ST1C2_RATSult1c2, Sultk1, RGD1562392Sulfotransferase 1C2ST1C2_RATquantification method: spectral counting
A572CXSTXB2_RATStxbp2, Unc18bSyntaxin-binding protein 2STXB2_RATquantification method: spectral counting
A1731 SYNJ2_RATSynj2Synaptojanin-2SYNJ2_RATquantification method: spectral counting
A5179 TBA1B_RATTuba1b, Tuba2Tubulin alpha-1B chainTBA1B_RATquantification method: spectral counting
A9106 THIO_RATTxn, Txn1ThioredoxinTHIO_RATquantification method: spectral counting
A7980 THNS2_RATThnsl2Threonine synthase-like 2THNS2_RATquantification method: spectral counting
A1469XTHRB_RATF2ProthrombinTHRB_RATquantification method: spectral counting
A708B TMM27_RATTmem27, Nx17CollectrinTMM27_RATquantification method: spectral counting
A0433 TPIS_RATTpi1Triosephosphate isomeraseTPIS_RATquantification method: spectral counting
A8015 TPMT_RATTpmt, OclnThiopurine S-methyltransferaseTPMT_RATquantification method: spectral counting
A8016XTPP1_RATTpp1, Cln2Tripeptidyl-peptidase 1TPP1_RATquantification method: spectral counting
A9509XTR11B_RATTnfrsf11b, OpgTumor necrosis factor receptor superfamily member 11BTR11B_RATquantification method: spectral counting
A643CXTRFE_RATTf, SrprbSerotransferrinTRFE_RATquantification method: spectral counting
A8037 TRM11_RATTrmt11tRNA (guanine(10)-N2)-methyltransferase homologTRM11_RATquantification method: spectral counting
A4534 TRPM8_RATTrpm8, Cmr1Transient receptor potential cation channel subfamily M member 8TRPM8_RATquantification method: spectral counting
A8047 TRXR1_RATTxnrd1, Trxr1Thioredoxin reductase 1TRXR1_RATquantification method: spectral counting
A5505 TXND3_RATNme8, Sptrx2, Txndc3Thioredoxin domain-containing protein 3TXND3_RATquantification method: spectral counting
A8135 UBR4_RATUbr4, Rbaf600, Zubr1E3 ubiquitin-protein ligase UBR4UBR4_RATquantification method: spectral counting
A4260XUFD1_RATUfd1lUbiquitin fusion degradation protein 1 homologUFD1_RATquantification method: spectral counting
A8170 UK114_RATHrsp12, Psp1Ribonuclease UK114UK114_RATquantification method: spectral counting
A472E UP1_RATRup2, LOC100359516Urinary protein 1UP1_RATquantification method: spectral counting
A473E UP2_RAT Urinary protein 2UP2_RATquantification method: spectral counting
A9139 UROM_RATUmodUromodulinUROM_RATquantification method: spectral counting
A0400 VATB2_RATAtp6v1b2, Atp6b2, Vat2V-type proton ATPase subunit BVATB2_RATquantification method: spectral counting
A0404 VATE1_RATAtp6v1e1V-type proton ATPase subunit E 1VATE1_RATquantification method: spectral counting
A0425XVDAC1_RATVdac1Voltage-dependent anion-selective channel protein 1VDAC1_RATquantification method: spectral counting
A8184 VGFR2_RATKdr, Flk1Vascular endothelial growth factor receptor 2VGFR2_RATquantification method: spectral counting
A9149XVTDB_RATGc, DbpVitamin D-binding proteinVTDB_RATquantification method: spectral counting
A5842 ASSY_RATAss1, AssArgininosuccinate synthaseASSY_RATquantification method: spectral counting
A857B CALB1_RATCalb1CalbindinCALB1_RATquantification method: spectral counting
A0008 CALM_RATCalm1, Calm2, Calm3CalmodulinCALM_RATquantification method: spectral counting

Compile date 08-10-2018© iMODE-CKD consortium