CKDdb-logoCKDdb - Study ID Exp22536212

PubMed ID22536212
AuthorsJin J, Ku YH, Kim Y, Kim Y, Kim K, Lee JY, Cho YM, Lee HK, Park KS, Kim Y.
TitleDifferential proteome profiling using iTRAQ in microalbuminuric and normoalbuminuric type 2 diabetic patients.
JournalExp Diabetes Res. 2012;2012:168602.
AbstractDiabetic nephropathy (DN) is a long-term complication of diabetes mellitus that leads to end-stage renal disease. Microalbuminuria is used for the early detection of diabetic renal damage, but such levels do not reflect the state of incipient DN precisely in type 2 diabetic patients because microalbuminuria develops in other diseases, necessitating more accurate biomarkers that detect incipient DN. Isobaric tags for relative and absolute quantification (iTRAQ) were used to identify urinary proteins that were differentially excreted in normoalbuminuric and microalbuminuric patients with type 2 diabetes where 710 and 196 proteins were identified and quantified, respectively. Some candidates were confirmed by 2-DE analysis, or validated by Western blot and multiple reaction monitoring (MRM). Specifically, some differentially expressed proteins were verified by MRM in urine from normoalbuminuric and microalbuminuric patients with type 2 diabetes, wherein alpha-1-antitrypsin, alpha-1-acid glycoprotein 1, and prostate stem cell antigen had excellent AUC values (0.849, 0.873, and 0.825, resp.). Moreover, we performed a multiplex assay using these biomarker candidates, resulting in a merged AUC value of 0.921. Although the differentially expressed proteins in this iTRAQ study require further validation in larger and categorized sample groups, they constitute baseline data on preliminary biomarker candidates that can be used to discover novel biomarkers for incipient DN.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection methodSample
Homo sapiensurinewholeDiabetes86hybrid quadrupole-TOF, LC-MS/MSDem22536212

Sample description and preparation

N (case)43
N (control)43
Disease (case)type 2 diabetic nephropathy
Disease (control)unhealthy_T2DM
Preparation specific methodiTRAQ labelling
Digestin-solution, trypsin

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)ValidationScores
A1526 OSTP_HUMANSPP1, BNSP, OPNOsteopontin precursorNP_000573.1 
A1276XCLUS_HUMANCLU, APOJ, CLIClusterin precursorNP_001822.2 
A5210 TFF3_HUMANTFF3, ITF, TFITrefoil factor 3 precursorNP_003217.2 
A9139 UROM_HUMANUMODUromodulin precursorNP_003352.1 
A344E SPRL1_HUMANSPARCL1, PIG33SPARC-like protein 1 precursorNP_004675.2 
A3849 K2C1_HUMANKRT1, KRTAKeratinNP_006112.2 
A8272 IGJ_HUMANIGJ, IGCJImmunoglobulin J chainNP_653247.1 
A4986 MUC1_HUMANMUC1, PUMMucin-1NP_877418.1 
A9787 NR3L1_HUMANNCR3LG1, B7H6Putative IG-like domain-containing protein DKFZP686O24166/DKFZP686I21167XP_370615.2 
A0609 EGF_HUMANEGFPro-epidermal growth factor precursorAAR84237.2 
A1581XALBU_HUMANALB, GIG20, GIG42Serum albumin precursorAAF01333.1 
A1742 HBB_HUMANHBB, beta-globinHemoglobin beta chainAAL68978.1 
A1939 HV102_HUMANIGH, VH1, IGHG1Immunoglobulin G1 Fab heavy chain variable regionAAR32503.1 
A4616 CAD13_HUMANCDH13, CDHHCadherin-13 precursorAAH30653.1 
A4856XHSPB1_HUMANHSPB1, HSP27, HSP28Heat shock protein beta-1AAA62175.1 
A5149 SPRR3_HUMANSPRR3, SPRCEsophaginAAH17802.1 
A6039 CEL_HUMANCEL, CELP, CELLBile salt-activated lipaseAAA52014.1 
A7381 PGRP2_HUMANPGLYRP2, PGLYRPL, PGRPLN-acetylmuramoyl-L-alanine amidase precursorAAQ88523.1 
A7540XPTGDS_HUMANPTGDS, PDS, PGDS2Prostaglandin-H2 D-isomerase precursorAAB27607.1 
A7843XSODE_HUMANSOD3Extracellular superoxide dismutase [Cu-Zn] precursorAAV40827.1 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainAAB50880.2 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainAAB53267.1 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainAAO11857.1 
A8364XLV605_HUMANIGL, IGLV3-22, V2-15Ig lambda chainAAD16731.1 
A9841 CMGA_HUMANCHGAChromogranin A precursorAAA52018.1 
A1411XIGHA1_HUMANIGM, SNC73, IGHA1Ig alpha-1 chain C regionp763134A 
A1548 EPCR_HUMANPROCR, EPCREndothelial protein C receptor precursorhCP1858145 
A1742 HBB_HUMANHBB, beta-globinHemoglobin beta chain1O1P_D 
A1939 HV102_HUMANIGH, VH1, IGHG1Immunoglobulin G1 Fab heavy chain variable regionp765044A 
A357B CD7_HUMANCD7T-cell antigen CD7 precursorhCP1778903.1 
A5448 REG1A_HUMANREG1A, PSPS, PSPS1Lithostathine 1 alpha precursor1QDD_A 
A5952 C1RL_HUMANC1RL, C1RL1, C1RLPComplement C1r-like proteinasehCP42501.1 
A648CXTTHY_HUMANTTR, PALB, TBPATransthyretin precursor5TTR_H 
A6589 GGH_HUMANGGHGamma-glutamyl hydrolase precursor1L9X_D 
A7843XSODE_HUMANSOD3Extracellular superoxide dismutase [Cu-Zn] precursorhCP51001.2 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainBAA19556.1 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chain1T04_C 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainA23746 
A8364XLV605_HUMANIGL, IGLV3-22, V2-15Ig lambda chainS12443 
A8369XA1AT_HUMANA1A, SERPINA1, AATAlpha-1-antitrypsin precursor1HP7_A 
A8381XAACT_HUMANSERPINA3, AACT, GIG24Alpha-1-antichymotrypsin precursorhCP1909255 
A8384XANT3_HUMANAT3, SERPINC1, PRO0309Antithrombin-III precursor1ATH_B 
A9038 SAP3_HUMANGM2AGanglioside GM2 activator precursorS13195 
A1080XCOIA1_HUMANCOL18A1, MFPCollagen alpha 1(XVIII) chain precursorCAB90482.1 
A1469XTHRB_HUMANF2Prothrombin precursorCAA23842.1 
A1712 TRFL_HUMANLTF, LF, GIG12Lactotransferrin precursorCAA37914.1 
A195AXZA2G_HUMANAZGP1, ZAG, ZNGP1Zinc-alpha-2-glycoprotein precursorCAA42438.1 
A5625XA1AG1_HUMANORM1, AGP1, ORM2Alpha-1-acid glycoprotein 1 precursorCAA29229.1 
A5626 A1AG2_HUMANORM2, AGP2, AGP-BAlpha-1-acid glycoprotein 2 precursorCAA29873.2 
A6004XCBPE_HUMANCPECarboxypeptidase E precursorBAA86053.1 
A7481 PPGB_HUMANCTSA, PPGBCathepsin ACAI20248.1 
A8269XIGHG2_HUMANIGHG2Ig gamma-2BAC85395.1 
A8269XIGHG2_HUMANIGHG2Ig gamma-2BAC85483.1 
A8270XIGHG3_HUMANIGHG3Ig gamma-3 chainCAA12585.1 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainBAB18261.1 
A8273XKV402_HUMANIGK, SDNK1, A30Ig kappa chainBAC01692.1 
A8364XLV605_HUMANIGL, IGLV3-22, V2-15Ig lambda chainCAA40946.1 
A8381XAACT_HUMANSERPINA3, AACT, GIG24Alpha-1-antichymotrypsin precursorCAA48671.1 
A7653 QSOX1_HUMANQSOX1, QSCN6, BPGF-1Sulfhydryl oxidase 1O00391 
A318B BT2A2_HUMANBTN2A2, BT2.2, BTF2ButyrophilinO00480 
A4679 CHL1_HUMANCHL1, CALLNeural cell adhesion molecule L1-like proteinO00533 
A4194 PSCA_HUMANPSCA, UNQ206/PRO232, UNQ206Prostate stem cell antigen precursorO43653 
A043A PGRP1_HUMANPGLYRP1, PGLYRP, PGRPPeptidoglycan recognition protein 1O75594 
A6042 CERU_HUMANCPCeruloplasmin precursorP00450method: Western blot
A1631XHPT_HUMANHPHaptoglobin precursorP00738method: Western blot
BC149 PEPA3_HUMANPGA3, PGA4Pepsin A-3P00790 
A8369XA1AT_HUMANA1A, SERPINA1, AATAlpha-1-antitrypsin precursorP01009method: Western blot
A1467XFIBA_HUMANFGAFibrinogen alpha/alpha-E chain precursorP02671 
A8663XAPOH_HUMANAPOH, B2G1Beta-2-glycoprotein 1 precursorP02749 
A8383XAMBP_HUMANAMBP, ITIL, HCPAMBP protein precursorP02760 
A5625XA1AG1_HUMANORM1, AGP1, ORM2Alpha-1-acid glycoprotein 1 precursorP02763method: Western blot
A8783XFETUA_HUMANAHSG, FETUA, PRO2743Alpha-2-HS-glycoprotein precursorP02765 
A1581XALBU_HUMANALB, GIG20, GIG42Serum albumin precursorP02768 
A9149XVTDB_HUMANGCVitamin D-binding protein precursorP02774method: Western blot
A643CXTRFE_HUMANTF, PRO1400Serotransferrin precursorP02787method: Western blot
A1712 TRFL_HUMANLTF, LF, GIG12Lactotransferrin precursorP02788 
A989B FRIL_HUMANFTL, FTLvariantFerritin light chainP02791 
A1471XVTNC_HUMANVTNVitronectin precursorP04004 
A735CXA1BG_HUMANA1BGAlpha-1B-glycoprotein precursorP04217 
A5802 AMY1_HUMANAMY1B, AMY1A, AMY1Alpha-amylase 1BP04745 
A5805XAMYP_HUMANAMY2A, AMY2BAlpha-amylase 2AP04746 
A1550 S10A8_HUMANS100A8, CAGA, CFAGCalgranulin AP05109 
A1549 IPSP_HUMANSERPINA5, PCI, PLANH3Plasma serine protease inhibitorP05154 
A1591XIC1_HUMANSERPING1, C1IN, C1NHSerine/cysteine proteinase inhibitor clade G member 1P05155 
A5448 REG1A_HUMANREG1A, PSPS, PSPS1Lithostathine 1 alpha precursorP05451 
A8515 THBG_HUMANSERPINA7, TBGThyroxine-binding globulin precursorP05543 
A1551 S10A9_HUMANS100A9, CAGB, CFAGProtein S100-A9P06702 
A790B ACBP_HUMANDBIAcyl-CoA-binding proteinP07108 
A0369 LDHB_HUMANLDHBL-lactate dehydrogenase B chainP07195 
A5984XCATD_HUMANCTSD, CPSDCathepsin D precursorP07339 
A9139 UROM_HUMANUMODUromodulin precursorP07911 
A7709 RNAS1_HUMANRNASE1, RIB1, RNS1Ribonuclease pancreatic precursorP07998 
A8390XCBG_HUMANSERPINA6, CBGCorticosteroid-binding globulin precursorP08185 
A5925 BGLR_HUMANGUSB, F8Beta-glucuronidase precursorP08236 
A7149 NEP_HUMANMME, EPNNeprilysinP08473 
A8706 CD14_HUMANCD14Monocyte differentiation antigen CD14 precursorP08571 
A357B CD7_HUMANCD7T-cell antigen CD7 precursorP09564 
A0706 PGFRB_HUMANPDGFRB, CSF1R, PDGFRBeta-type platelet-derived growth factor receptor precursorP09619 
A7710 RNAS2_HUMANRNASE2, EDN, RNS2Nonsecretory ribonuclease precursorP10153 
A1526 OSTP_HUMANSPP1, BNSP, OPNOsteopontin precursorP10451 
A8522 UTER_HUMANSCGB1A1, CC10, CCSPClara cell phospholipid-binding protein precursorP11684 
A4647 CD99_HUMANCD99, MIC2, MIC2XT-cell surface glycoprotein E2 precursorP14209 
A1933 PVR_HUMANPVR, PVSPoliovirus receptor precursorP15151 
A2489 GNS_HUMANGNSN-acetylglucosamine-6-sulfatase precursorP15586 
A8274 IGLL1_HUMANIGLL1, IGL1, IGLJ14.1Immunoglobulin lambda-like polypeptide 1 precursorP15814 
A5923 BGAL_HUMANGLB1, ELNR1Beta-galactosidase precursorP16278 
A1564 VCAM1_HUMANVCAM1, L1CAMVascular cell adhesion protein 1 precursorP19320 
A4646 CD9_HUMANCD9, BTCC-1, MIC3CD9 antigenP21926 
A6644 GPX3_HUMANGPX3, GPXPGlutathione peroxidase 3P22352 
A1544 PROZ_HUMANPROZVitamin K-dependent protein Z precursorP22891 
A1001 DNAS1_HUMANDNASE1, DNL1, DRNIDeoxyribonuclease 1P24855 
A0808XMOES_HUMANMSNMoesinP26038 
A1609 CALR_HUMANCALR, CRTCCalreticulin precursorP27797 
A3829 K1C9_HUMANKRT9KeratinP35527 
A3852XK22E_HUMANKRT2, KRT2A, KRT2EKeratinP35908 
A7540XPTGDS_HUMANPTGDS, PDS, PGDS2Prostaglandin-H2 D-isomerase precursorP41222 
A795BXAFAM_HUMANAFM, ALB2, ALBAAfamin precursorP43652 
A4951XLUM_HUMANLUM, LDC, SLRR2DLumican precursorP51884 
A5806 ANAG_HUMANNAGLU, UFHSD1, ufHSD2Alpha-N-acetylglucosaminidaseP54802 
A4625 CADH6_HUMANCDH6Cadherin-6 precursorP55285 
A4616 CAD13_HUMANCDH13, CDHHCadherin-13 precursorP55290 
A0210 NTF2_HUMANNUTF2, NTF2, PP15Nuclear transport factor 2P61970 
A3574XBASP1_HUMANBASP1, NAP22Brain acid soluble protein 1P80723 
A1555XPGBM_HUMANHSPG2Basement membrane-specific heparan sulfate proteoglycan core proteinP98160 
A6335 DIAC_HUMANCTBS, CTBDi-N-acetylchitobiase precursorQ01459 
A0441 FABP5_HUMANFABP5Fatty acid binding protein 5 (psoriasis-associated)Q01469 
A5935 BMPR2_HUMANBMPR2, PPH1Bone morphogenetic protein receptor type II (serine/threonine kinase) precursorQ13873 
A0008 CALM_HUMANCALM1, CALM2, CALM3CalmodulinQ13942 
A8434XITIH4_HUMANITIH4, IHRP, ITIHL1Inter-alpha-trypsin inhibitor heavy chain H4 precursorQ14624 
A4857XIBP7_HUMANIGFBP7, MAC25, PSFInsulin-like growth factor binding protein 7 precursorQ16270 
A1497XUROK_HUMANATF, PLAUUrokinase-type plasminogen activator precursorQ5SWW9 
A278E S10A7_HUMANS100A7, PSOR1, S100A7CProtein S100-A7Q5SY67 
A1606 MASP2_HUMANMASP2, MASP-2Mannan-binding lectin serine protease 2Q5TEQ5 
A180E UBC_HUMANUBC, UBC1Polyubiquitin UBCQ5UGI3 
A925BXCUBN_HUMANCUBN, IFCRCubilinQ5VTA6 
A1555XPGBM_HUMANHSPG2Basement membrane-specific heparan sulfate proteoglycan core proteinQ5VU27 
A1614 DAF_HUMANCD55, DAF, CRComplement decay-accelerating factor precursorQ5VW91 
A353CXRET4_HUMANRBP4, PRO2222, PRBPRetinol binding protein 4Q5VY30 
A2407 COCA1_HUMANCOL12A1, COL12A1LCollagen alpha 1(XII) chain precursorQ5VYK1 
A471B GOLM1_HUMANGOLPH2, GOLM1, UNQ686/PRO1326Golgi membrane protein 1Q5VZE3 
A3786 K1C13_HUMANGUCA1B, KRT13KeratinQ6AZK5 
A224C NGAL_HUMANLCN2, HNL, NGALNeutrophil gelatinase-associated lipocalin precursorQ6FGL5 
A1411XIGHA1_HUMANIGM, SNC73, IGHA1Ig alpha-1 chain C regionQ6GMX2 
A1819XB2MG_HUMANB2M, HDCMA22PBeta-2-microglobulin precursorQ6IAT8 
A5626 A1AG2_HUMANORM2, AGP2, AGP-BAlpha-1-acid glycoprotein 2 precursorQ6IB74 
A9038 SAP3_HUMANGM2AGanglioside GM2 activator precursorQ6LBL5 
A6859 KLK3_HUMANKLK3, APSProstate specific antigen precursorQ6LDS3 
A8269XIGHG2_HUMANIGHG2Ig gamma-2Q6MZU6 
A1466XFINC_HUMANFN1, FNFibronectinQ6N025 
A1539XKNG1_HUMANKNG1, BDK, KNGKininogen-1Q6PAU9 
A9298 FCG3A_HUMANFCGR3A, CD16A, FCG3Low affinity immunoglobulin gamma Fc region receptor III-A precursorQ6PIJ0 
A1177 A2MG_HUMANA2M, CPAMD5, FWP007Alpha-2-macroglobulin precursorQ6PN97 
A2490 SULF2_HUMANSULF2, UNQ559/PRO1120, UNQ559Extracellular sulfatase Sulf-2 precursorQ6UX86 
A8454 PI16_HUMANPI16, CRISP9, PSPBPPeptidase inhibitor 16 precursorQ6UXB8 
A3682 ODO2_HUMANDLST, DLTS, E2Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complexQ7LDY7 
A3846 K2C6C_HUMANKRT6C, KRT6E, KRT6DKeratinQ7RTN9 
A182A VMO1_HUMANVMO1, UNQ6350/PRO21055, UNQ6350Vitteline membrane outer layer protein 1 homolog precursorQ7Z5L0 
A1410XCO6A1_HUMANCOL6A1Collagen alpha 1(VI) chain precursorQ7Z645 
A553F C1QL4_HUMANC1QL4, CTRP11Complement C1Q-like protein 4 precursorQ86Z23 
A5065 PCDH1_HUMANPCDH1Protocadherin 1 precursorQ8IUP2 
A1322XPIGR_HUMANPIGRPolymeric immunoglobulin receptor precursorQ8IZY7 
A3824 K1C10_HUMANKRT10, KPPKeratinQ8N175 
A1935 CADM1_HUMANCADM1, IGSF4, IGSF4ACell adhesion molecule 1 precursorQ8N2F4 
A1491XCO1A1_HUMANCOL1A1Collagen alpha-1(I) chainQ8N473 
A195AXZA2G_HUMANAZGP1, ZAG, ZNGP1Zinc-alpha-2-glycoprotein precursorQ8N4N0 
A8454 PI16_HUMANPI16, CRISP9, PSPBPPeptidase inhibitor 16 precursorQ8NBK0 
A4609 C99L2_HUMANCD99L2, MIC2L1, UNQ1964/PRO4486CD99 antigen-like 2Q8TCZ2 
A5983 CATC_HUMANCTSC, CPPIDipeptidyl-peptidase 1 precursorQ8WY99 
A9469 ROBO4_HUMANROBO4, UNQ421/PRO3674, UNQ421Roundabout homolog 4 precursorQ8WZ75 
A1930XPVRL2_HUMANPVRL2, HVEB, PRR2Poliovirus receptor related protein 2 precursorQ92692 
A6589 GGH_HUMANGGHGamma-glutamyl hydrolase precursorQ92820 
A4614 CAD11_HUMANCDH11Cadherin-11 precursorQ96CZ9 
A6477 F16P1_HUMANFBP1, FBPFructose-1Q96E46 
A560B P3IP1_HUMANPIK3IP1, HGFLPhosphoinositide-3-kinase-interacting protein 1Q96FE7 
A8443 NEUS_HUMANSERPINI1, PI12Neuroserpin precursorQ99574 
A1741 HBA_HUMANHBA1, HBA2, HBZHemoglobin subunit alphaQ9BX83 
A9282 COL12_HUMANCOLEC12, CLP1, NSR2Scavenger receptor with C-type lectin type IQ9BYH7 
A9282 COL12_HUMANCOLEC12, CLP1, NSR2Scavenger receptor with C-type lectin type IQ9BYH7 
A9282 COL12_HUMANCOLEC12, CLP1, NSR2Scavenger receptor with C-type lectin type IQ9BYH7 
A721D MMRN2_HUMANMMRN2, EMILIN3Multimerin-2Q9H8L6 
A353B CD248_HUMANCD248, CD164L1, TEM1Tumor endothelial marker 1 precursorQ9HCU0 
A7819 SIAE_HUMANSIAE, YSG2, CSE-CSialate O-acetylesteraseQ9NT71 
A760B YIPF3_HUMANYIPF3, KLIP1Protein YIPF3Q9NWE3 
A760B YIPF3_HUMANYIPF3, KLIP1Protein YIPF3Q9NWE3 
A760B YIPF3_HUMANYIPF3, KLIP1Protein YIPF3Q9NWE3 
A9331 GPC5B_HUMANGPRC5B, RAIG2G protein-coupled receptorQ9NZH0 
A9846 CRNN_HUMANCRNN, DRC1, PDRC1CornulinQ9UBG3 
A6853XKLK1_HUMANKLK1, KALLIKREINKallikrein 1Q9UD19 
A0318XCADH1_HUMANCDH1, CDHE, UVOEpithelial-cadherin precursorQ9UII8 
A0821 CD44_HUMANCD44, LHR, MDU2CD44 antigenQ9UJ36 
A1606 MASP2_HUMANMASP2, MASP-2Mannan-binding lectin serine protease 2Q9UMV3 
A9399 LYVE1_HUMANLYVE1, CRSBP1, HARLymphatic vessel endothelial hyaluronic acid receptor 1 precursorQ9UNF4 
A760B YIPF3_HUMANYIPF3, KLIP1Protein YIPF3Q9Y3U9 
A4711 COFA1_HUMANCOL15A1Collagen alpha 1(XV) chain precursorQ9Y4W4 
A7367 CBPQ_HUMANCPQ, PGCP, LCH1Blood plasma glutamate carboxypeptidase precursorQ9Y5X6 
A9399 LYVE1_HUMANLYVE1, CRSBP1, HARLymphatic vessel endothelial hyaluronic acid receptor 1 precursorQ9Y5Y7 

Compile date 08-10-2018© iMODE-CKD consortium