CKDdb-logoCKDdb - Study ID Exp20007950a

PubMed ID20007950
AuthorsYohannes E, Chang J, Tar MT, Davies KP, Chance MR.
TitleMolecular targets for diabetes mellitus-associated erectile dysfunction.
JournalMol Cell Proteomics. 2010 Mar;9(3):565-78.
AbstractProtein expression profiles in rat corporal smooth muscle tissue were compared between animal models of streptozotocin-induced diabetes mellitus (STZ-DM) and age-matched controls (AMCs) at 1 week and 2 months after induction of hyperglycemia with STZ treatment. At each time point, protein samples from four STZ-DM and four AMC rat corpora tissues were prepared independently and analyzed together across multiple quantitative two-dimensional gels using a pooled internal standard sample to quantify expression changes with statistical confidence. A total of 170 spots were differential expressed among the four experimental groups. A subsequent mass spectrometry analysis of the 170 spots identified a total of 57 unique proteins. Network analysis of these proteins using MetaCore suggested altered activity of transcriptional factors that are of too low abundance to be detected by the two-dimensional gel method. The proteins that were down-regulated with diabetes include isoforms of collagen that are precursors to fibril-forming collagen type 1; Hsp47, which assists and mediates the proper folding of procollagen; and several proteins whose abundance is controlled by sex hormones (e.g. CRP1 and A2U). On the other hand, proteins seen or predicted to be up-regulated include proteins involved in cell apoptosis (e.g. p53, 14-3-3-gamma, Serpinf1, Cct4, Cct5, and Sepina3n), proteins that neutralize the biological activity of nerve growth factor (e.g. anti-NGF 30), and proteins involved in lipid metabolism (e.g. apoA-I and apoA-IV). Subsequent Western blot validation analysis of p53, 14-3-3-gamma, and Hsp47 confirmed increased p53 and 14-3-3-gamma and decreased Hsp47 levels in separate samples. According to the results from the Western blot analysis, Hsp47 protein showed a approximately 3-fold decrease at 1 week and was virtually undetectable at 2 months in diabetic versus control. Taken together, our results identify novel candidate proteins playing a role in erectile dysfunction in diabetes resulting from STZ treatment.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection method
Rattus norvegicuscorporawholeDiabetes8MALDI-TOF, LC-MS/MS

Sample description and preparation

N (case)4
N (control)4
Disease (case)Diabetes Mellitus-associated Erectile Dysfunction
Disease (control)healthy, intrap. inj. citrate buffer
Disease inductionintrap. inj. STZ (35 mg/kg), 1w
Digestin-gel, trypsin

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)Scores
Q8K1Q6 Q8K1Q6LOC298116, LOC298109Alpha-2u globulin PGCL266730407ratio: 0.21
A1581XALBU_RATAlbSerum albumin ( Precursor)P002770ratio: 1.34
A8367 22P1_RATAndpro, Crp1Cystatin-related protein 1P22282ratio: 0.43
A1597 SAMP_RATApcs, Ptx2, SapSerum amyloid P-componentP23680ratio: 2.04
A5683 ACPH_RATApehAcylamino-acid-releasing enzymeP13676ratio: 1.21
A1552 APOA1_RATApoa1Apolipoprotein A-IP04639ratio: 2.89
A810BXAPOA4_RATApoa4Apolipoprotein A-IVP02651ratio: 2.24
A1176 APOE_RATApoeApolipoprotein EP02650ratio: 0.51
A7442 Q6P6G4Bpgm2,3-bisphosphoglycerate mutaseQ6P6G4ratio: 1.58
A1598XCO3_RATC3Complement C3P01026ratio: 1.63
A1616 CO5_RATC5Complement C51.09E+08ratio: 1.2
A5964XCAH2_RATCa2Carbonic anhydrase 2P27139ratio: 1.67
A5959 CAH1_RATCa1, Car1Carbonic anhydrase 127689031ratio: 1.72
A643CXTRFE_RATTfSerotransferrin33086660ratio: 0.58
A3538XTCPD_RATCct4T-complex protein 1 subunit delta50927753ratio: 1.42
A3543XQ6B436Cct5Cct-5 protein ( Fragment)Q6B436ratio: 1.27
A4709XD3ZZT9Col14a1Protein Col14a11.09E+08ratio: 0.9
A1491XCO1A1_RATCol1a1Collagen alpha-1(I) chainP02454ratio: 0.6
A1492XCO1A2_RATCol1a2Collagen alpha-2(I) chainP02466ratio: 0.51
A1422 Q5EB88Col6a2Col6a2 protein ( Fragment)Q5EB88ratio: 0.66
A1423XD4A115Col6a3Protein Col6a31.58E+08ratio: 1.32
A3608 DPYL3_RATDpysl3, Crmp4Dihydropyrimidinase-related protein 314518293ratio: 1.48
A6466 B0BNJ4Ethe1, Ethe1_predictedEthylmalonic encephalopathy 1B0BNJ4ratio: 1.21
A462D FETUB_RATFetub, FetubFetuin-BQ9QX79ratio: 1.51
A1467XFIBA_RATFgaFibrinogen alpha chainP06399ratio: 1.34
A1493 FIBB_RATFgbFibrinogen beta chainP14480ratio: 1.37
A1494XFIBG_RATFggFibrinogen gamma chain ( Precursor)P02680ratio: 1.31
A424AXD3ZWR5Fhl1Four and a half LIM domains 14894849ratio: 0.98
A1215XD3ZAN3Ganab, Ganab_predictedAlpha glucosidase 2 alpha neutral subunit (Predicted)1.09E+08ratio: 1.51
A0515 GUAD_RATGdaGuanine deaminase7533042ratio: 1.08
A6644 GPX3_RATGpx3Glutathione peroxidase 3P23764ratio: 1.73
A6668 GSTA6_RATGsta6Glutathione S-transferase A6Q6AXY0ratio: 0.59
A3683 Q99521HadhaGastrin-binding protein ( Fragment)1.49E+08ratio: 1.22
A016C HBB1_RATHbbHemoglobin subunit beta-1P02091ratio: 1.68
A8482 SERPH_RATSerpinh1, Cbp1, Hsp47Serpin H1P29457ratio: 0.34
A1631XHPT_RATHpHaptoglobinP06866ratio: 0.88
A021CXHEMO_RATHpxHemopexin ( Precursor)P20059ratio: 1.52
Igc  Igcanti-NGF30 antibody lightchain4096754ratio: 1.86
A1517XLAMB2_RATLamb2Laminin subunit beta-2P15800ratio: 0.58
A0369 LDHB_RATLdhb, Ldh-2, Ldh2L-lactate dehydrogenase B chainP42123ratio: 1.57
A1374XLMNA_RATLmna, Lmn1Prelamin-A/CP48679ratio: 1.15
A1128 Q6P7A6Smad1SMAD family member 11.09E+08ratio: 1.26
A9969 KNT1_RATMap1T-kininogen 1P01048ratio: 2.29
A4998 MYP0_RATMpz, P0Myelin protein P0P06907ratio: 1.99
A777D D3ZGY2Otud6b, Otud6b_predictedOTU domain containing 6B (Predicted)1.58E+08ratio: 1.37
A5096 PRELP_RATPrelpProlargin47938987ratio: 0.45
A0244 PSB4_RATPsmb4Proteasome subunit beta type-4P34067ratio: 1.19
A0583 PUF60_RATPuf60, Siahbp1Poly(U)-binding-splicing factor PUF60Q9WV25ratio: 1.87
A8377 A1M_RATA1m, PzpAlpha-1-macroglobulinQ63041ratio: 2.19
A8369XA1AT_RATSerpina1Alpha-1-antiproteinaseP17475ratio: 2.33
A8494 SPA3N_RATSerpina3n, Spin2cSerine protease inhibitor A3NP09006ratio: 1.84
A8448XQ80ZA3Serpinf1, Dmrs91Alpha-2 antiplasminQ80ZA3ratio: 1.61
sid23p  sid23psimilar to sid23p27703460ratio: 0.93
A3213XSMC3_RATSmc3, Bam, BmhStructural maintenance of chromosomes protein 3P97690ratio: 0.84
A512C SNX1_RATSnx1Sorting nexin-1Q99N27ratio: 1.11
A643CXTRFE_RATTfSerotransferrinP12346ratio: 1.59
A0362 1433G_RATYwhag14-3-3 protein gammaP61983ratio: 1.97

Compile date 08-10-2018© iMODE-CKD consortium