CKDdb-logoCKDdb - Study ID Exp17881425

PubMed ID17881425
AuthorsShui HA, Huang TH, Ka SM, Chen PH, Lin YF, Chen A.
TitleUrinary proteome and potential biomarkers associated with serial pathogenesis steps of focal segmental glomerulosclerosis.
JournalNephrol Dial Transplant. 2008 Jan;23(1):176-85. Epub 2007 Sep 19.
AbstractBACKGROUND: Focal segmental glomerulosclerosis (FSGS) is a chronic nephropathy showing characteristic glomerular sclerosis. So far, the diagnosis and prognosis of FSGS rely mainly on the invasive biopsy. Searching for potential FSGS-associated urinary biomarkers representing pre-sclerotic and serial sclerotic stages of FSGS could be helpful to the non-invasive diagnosis and prognosis of FSGS. METHODS: In the present study, we used a 2D gel-based proteomic approach to identify urinary proteins at pre-sclerotic and different sclerotic stages of an FSGS mouse model in order to find FSGS-related urinary proteins. The FSGS mouse model was established in Balb/c mice by a single injection of adriamycin, and disease severity was monitored by renal biological parameters and histopathological features. Urine was collected on days 0, 4, 7, 11, 15 and 20, and subjected to two-dimensional electrophoresis (2-DE) analysis. Proteins were identified by matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) and a protein database search. Some of the identified proteins were confirmed by western blot analysis. RESULTS: We identified 37 urinary proteins showing characteristic patterns of dynamic changes along the disease course of FSGS. Early urinary proteins appearing before glomerular sclerosis were noticed. Importantly, 11 urine proteins are novel to FSGS and have known functions highly associated with different pathogenetic steps of the disease, including haemodynamic disturbance, podocyte apoptosis, ECM-protein deposition and glomerular sclerosis. CONCLUSIONS: Some urinary proteins appearing earlier than glomerular sclerosis could serve as potential early diagnostic biomarkers. The proteins with the pathogenic roles could serve as potential non-invasive prognostic markers of FSGS, and give an insight into pathogenic mechanisms of this sclerosis disease.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection method
Mus musculusurinewholeGlomerulonephritis12MALDI-TOF-MS

Sample description and preparation

Disease (case)focal segmental glomerulosclerosis
Disease inductionadriamycin, 0.1 mg/10 g b.w.
Digestin-gel, trypsin
Separation2-DE

Molecule list

Molecule IDextExternal IDGeneNameSource acc
A1539XKNG1_MOUSEKng1, KngKininogen-1O08677
A8694 CALU_MOUSECaluCalumeninO35887
A9832 CER1_MOUSECer1, Cerl, Cerr1CerberusO55233
A1581XALBU_MOUSEAlb, Alb-1, Alb1Serum albumin ( Precursor)P07724
A0318XCADH1_MOUSECdh1Cadherin-1P09803
A0647XANXA1_MOUSEAnxa1, Anx1, Lpc-1Annexin A1P10107
A1954XGSTM1_MOUSEGstm1Glutathione S-transferase Mu 1P10649
A150CXMUP1_MOUSEMup1, Mup10, Mup3Major urinary protein 1P11588
A151CXMUP2_MOUSEMup2Major urinary protein 2P11589
A6853XKLK1_MOUSEKlk1, Klk-6, Klk6Kallikrein-1P15947
A3488 B3A3_MOUSESlc4a3, Ae3Anion exchange protein 3P16283
A5796 AMPE_MOUSEEnpepGlutamyl aminopeptidaseP16406
A7403 PIMT_MOUSEPcmt1Protein-L-isoaspartate(D-aspartate) O-methyltransferaseP23506
A6465 EST1C_MOUSECes1c, Es1Carboxylesterase 1CP23953
A0778 MAP4_MOUSEMap4, Mtap4Microtubule-associated protein 4P27546
A4356 NPL4_MOUSENploc4, Kiaa1499, Npl4Nuclear protein localization protein 4 homologP60670
A8371 A1AT3_MOUSESerpina1c, Dom3, Dom6Alpha-1-antitrypsin 1-3P81105
A8804 GDIB_MOUSEGdi2, Gdi3Rab GDP dissociation inhibitor betaQ3TIY6
A9660 RT09_MOUSEMrps928S ribosomal protein S9Q3UMV5
A152C MUP3_MOUSEMup3, Mup25, Mup6Major urinary protein 3Q5D068
A5319 ECM1_MOUSEEcm1Extracellular matrix protein 1Q61508
A1584XCO4A1_MOUSECol4a1Collagen alpha-1(IV) chainQ6PHB5
A9097 TBCD5_MOUSETbc1d5TBC1 domain family member 5Q80XQ2
A6973XMAST4_MOUSEMast4, unknownMicrotubule-associated serine/threonine-protein kinase 4Q8BME3
A6482 FA2H_MOUSEFa2h, FaahFatty acid 2-hydroxylaseQ8BTH1
A667C VA0D2_MOUSEAtp6v0d2V-type proton ATPase subunit d 2Q8BTL3
A1581XALBU_MOUSEAlb, Alb-1, Alb1Serum albumin ( Precursor)Q8CG74
A5749 AIFM2_MOUSEAifm2, AmidApoptosis-inducing factor 2Q8CHZ2
A5263XADA32_MOUSEAdam32Disintegrin and metalloproteinase domain-containing protein 32Q8K410
A6215 CSAD_MOUSECsadCysteine sulfinic acid decarboxylaseQ8K566
A0241 MAPK2_MOUSEMapkapk2, Rps6kc1MAP kinase-activated protein kinase 2Q8R3U8
A643CXTRFE_MOUSETf, TrfSerotransferrinQ921I1
A5750 AK1A1_MOUSEAkr1a1, Akr1a4Alcohol dehydrogenase [NADP(+)]Q9JII6
A146A TEFF1_MOUSETmeff1Tomoregulin-1Q9JJS1
A5218 TMOD4_MOUSETmod4Tropomodulin-4Q9JLH8
A6464 ESTD_MOUSEEsd, Es10, Sid478S-formylglutathione hydrolaseQ9R0P3
A3542XTCPQ_MOUSECct8, CctqT-complex protein 1 subunit thetaQ9WVS5

Compile date 08-10-2018© iMODE-CKD consortium