CKDdb-logoCKDdb - Study ID Exp16372274

PubMed ID16372274
AuthorsPark MR, Wang EH, Jin DC, Cha JH, Lee KH, Yang CW, Kang CS, Choi YJ.
TitleEstablishment of a 2-D human urinary proteomic map in IgA nephropathy.
JournalProteomics. 2006 Feb;6(3):1066-76.
AbstractImmunoglobulin A nephropathy (IgAN) is the most common form of immune complex-mediated glomerulonephritis worldwide. Although chronic renal failure develops in considerable numbers of IgAN patients, the exact etiology has not yet been clearly elucidated. To establish the urinary protein map of IgAN, we performed a urinary proteomic analysis. Thirteen patients with IgAN and 12 normal controls were recruited. Morning midstream spot urine samples were used with Centriprep ultrafiltration for concentration and desalting. 2-DE was performed and compared between IgAN and normal control, and urinary proteins were identified by MALDI-TOF MS. A large number of protein spots were identified in IgAN and normal control samples, with means of 311 spots and 174 spots, respectively. Approximately 216 protein spots were detected as differentially expressed in IgAN. Among these, 82 spots were over-expressed, and 134 spots were under-expressed compared to normal controls. A total of 84 differentially expressed spots, representing 59 different proteins, were finally identified in IgAN. We have established a urinary proteomic map of IgAN and this result helps in the identification. Further study is needed to determine the potential pathogenic role of these proteins.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseNDetection method
Homo sapiensurinewholeGlomerulonephritis25MALDI-TOF-MS

Sample description and preparation

Disease (case)IgA nephropathy
Digestin-gel, trypsin
Separation2-DE

Molecule list

Molecule IDextExternal IDGeneNameSource accRegulation (case/control)Scores
A3551 PDE2A_HUMANPDE2AcGMP-dependent 3'O00408
A7445 PMM2_HUMANPMM2Phosphomannomutase 2O15305
A2995 Z324A_HUMANZNF324, ZNF324AZinc finger protein 324AO75467
A2995 Z324A_HUMANZNF324, ZNF324AZinc finger protein 324AO75467
A3564 NDUS3_HUMANNDUFS3NADH dehydrogenase [ubiquinone] iron-sulfur protein 3O75489
A0526 GRAP2_HUMANGRAP2, GADS, GRB2LGRB2-related adaptor protein 2O75791
A1539XKNG1_HUMANKNG1, BDK, KNGKininogen-1P01042
A1539XKNG1_HUMANKNG1, BDK, KNGKininogen-1P01042
A1539XKNG1_HUMANKNG1, BDK, KNGKininogen-1P01042
A1539XKNG1_HUMANKNG1, BDK, KNGKininogen-1P01042
A1741 HBA_HUMANHBA1, HBA2, HBZHemoglobin subunit alphaP01922
A1742 HBB_HUMANHBB, beta-globinHemoglobin beta chainP02023
A1742 HBB_HUMANHBB, beta-globinHemoglobin beta chainP02023
A1841 HBE_HUMANHBE1, HBEHemoglobin epsilon chainP02100
A8383XAMBP_HUMANAMBP, ITIL, HCPAMBP protein precursorP02760
A8383XAMBP_HUMANAMBP, ITIL, HCPAMBP protein precursorP02760
A8383XAMBP_HUMANAMBP, ITIL, HCPAMBP protein precursorP02760
A1581XALBU_HUMANALB, GIG20, GIG42Serum albumin precursorP02768
A1581XALBU_HUMANALB, GIG20, GIG42Serum albumin precursorP02768
A1581XALBU_HUMANALB, GIG20, GIG42Serum albumin precursorP02768
A643CXTRFE_HUMANTF, PRO1400Serotransferrin precursorP02787
A643CXTRFE_HUMANTF, PRO1400Serotransferrin precursorP02787
A643CXTRFE_HUMANTF, PRO1400Serotransferrin precursorP02787
A643CXTRFE_HUMANTF, PRO1400Serotransferrin precursorP02787
A5805XAMYP_HUMANAMY2A, AMY2BAlpha-amylase 2AP04746
A0195 INSR_HUMANINSRInsulin receptorP06213
A7915XSYEP_HUMANEPRS, GLNS, PARSBifunctional aminoacyl-tRNA synthetaseP07814
A5982XCATB_HUMANCTSB, CPSB, APPSCathepsin B precursorP07858
A7843XSODE_HUMANSOD3Extracellular superoxide dismutase [Cu-Zn] precursorP08294
A0428 ALDOC_HUMANALDOC, ALDCFructose-bisphosphate aldolase CP09972
A3198 MYH8_HUMANMYH8Myosin-8P13535
A1821 1A25_HUMANHLA-A, HLA-A*24, HLAHLA class I histocompatibility antigenP16188
A1350 IF2B_HUMANEIF2S2, EIF2BEukaryotic translation initiation factor 2 subunit 2P20042
A0912 TNAP3_HUMANTNFAIP3, OTUD7CTumor necrosis factorP21580
A7556 PUR6_HUMANPAICS, ADE2, AIRCMultifunctional protein ADE2P22234
A8229 HV106_HUMANIgG VH, IGHV7-81, IGHV1Ig heavy chain V-I regionP23083
A195AXZA2G_HUMANAZGP1, ZAG, ZNGP1Zinc-alpha-2-glycoprotein precursorP25311
A195AXZA2G_HUMANAZGP1, ZAG, ZNGP1Zinc-alpha-2-glycoprotein precursorP25311
A0265 MK03_HUMANMAPK3, ERK1, PRKM3Mitogen-activated protein kinase 3P27361
A3542XTCPQ_HUMANCCT8, CCTQT-complex protein 1P50990
A1672XCO4A4_HUMANCOL4A4Collagen alpha 4(IV) chain precursorP53420
A884B CLH2_HUMANCLTCL1, CLH22, CLTCLClathrin heavy chain 2P53675
A814B APOC4_HUMANAPOC4Apolipoprotein C-IV precursorP55056
A243C NU107_HUMANNUP107Nuclear pore complex protein Nup107P57740
A8642 2ABA_HUMANPPP2R2ASerine/threonine protein phosphatase 2AQ00007
A9455 PPARD_HUMANNR1C2, PPARD, PPARBPeroxisome proliferator activated receptor deltaQ03181
A2659 ZN155_HUMANZNF155, ZFP95Zinc finger protein 155Q12901
A813A OLIG2_HUMANOLIG2, BHLHB1, BHLHE19Oligodendrocyte transcription factor 2Q13516
A0646 ARHG7_HUMANARHGEF7, COOL1, P85SPRRho guanine nucleotide exchange factor 7Q14155
A3605XDPYL1_HUMANCRMP1, DPYSL1, ULIP3Dihydropyrimidinase related protein-1Q14194
A323C RAB35_HUMANRAB35, RAB1C, RAYRas-related protein Rab-35Q15286
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A4687XCNN3_HUMANCNN3Calponin 3Q15417
A8047 TRXR1_HUMANTXNRD1, TR, TR1Thioredoxin reductase 1Q16881
A5454 S1A7A_HUMANS100A7A, S100A15, S100A7L1S100 calcium-binding protein A15Q86SG5
A5454 S1A7A_HUMANS100A7A, S100A15, S100A7L1S100 calcium-binding protein A15Q86SG5
A625D KBTB3_HUMANKBTBD3, BKLHD3BTB and Kelch domain containing protein 3Q8NAB2
A2454 SYNE2_HUMANSYNE2, NUA, TROPHNesprin 2Q8WXH0
A8675 ASB13_HUMANASB13Ankyrin repeat and SOCS box containing protein 13Q8WXK3
A0713 P55G_HUMANPIK3R3Phosphatidylinositol 3-kinase regulatory gamma subunitQ92569
A0713 P55G_HUMANPIK3R3Phosphatidylinositol 3-kinase regulatory gamma subunitQ92569
A092A RHES_HUMANRASD2, TEM2GTP-binding protein RhesQ96D21
A0151 SIPA1_HUMANSIPA1, SPA1Signal-induced proliferation-associated protein 1Q96FS4
A2342XMACF1_HUMANMACF1, ABP620, ACF7Microtubule-actin crosslinking factor 1Q96PK2
A2342XMACF1_HUMANMACF1, ABP620, ACF7Microtubule-actin crosslinking factor 1Q96PK2
A3951 SFXN1_HUMANSFXN1Sideroflexin 1Q9H9B4
A2668 ZN160_HUMANZNF160, HZF5, KR18Zinc finger protein Kr18Q9HCG1
A1430 DPOD4_HUMANPOLD4, POLDSDNA polymerase delta subunit 4Q9HCU8
A411A EXOS3_HUMANEXOSC3, PNAS-3, RRP40Exosome complex exonuclease RRP40Q9NQT5
A7846 SPAST_HUMANSPAST, ADPSP, FSP2SpastinQ9UBPO
A3934 VTI1B_HUMANVTI1B, VTI1, VTI1LVesicle transport through interaction with t-SNAREs homolog 1BQ9UEUO
A2658 ZN221_HUMANZNF221, ZFP95Zinc finger protein 221Q9UK13
A0469 CTND2_HUMANCTNND2, NPRAPCatenin delta-2Q9UQB3
A928A RCL1_HUMANRCL1, RNAC, RPC2RNA 3'-terminal phosphate cyclase-like protein 1Q9Y2P8
A322E SLMO2_HUMANSLMO2, CGI-107Protein Slowmo homolog 2Q9Y3B1
A322E SLMO2_HUMANSLMO2, CGI-107Protein Slowmo homolog 2Q9Y3B1
A6826 KAD5_HUMANAK5Adenylate kinase 5Q9Y6K8

Compile date 08-10-2018© iMODE-CKD consortium