CKDdb-logoCKDdb - Study ID Exp15760396

PubMed ID15760396
AuthorsSchaub S, Wilkins JA, Antonovici M, Krokhin O, Weiler T, Rush D, Nickerson P.
TitleProteomic-based identification of cleaved urinary beta2-microglobulin as a potential marker for acute tubular injury in renal allografts.
JournalAm J Transplant. 2005 Apr;5(4 Pt 1):729-38.
AbstractOur aim is to develop noninvasive tests to monitor the renal allograft posttransplant. Previously, we have reported that an unbiased proteomic-based approach can detect urine protein peaks associated with acute tubulointerstitial renal allograft rejection. Identification of these proteins peaks by mass spectrometry demonstrated that they all derive from nontryptic cleaved forms of beta2-microglobulin. In vitro experiments showed that cleavage of intact beta2-microglobulin requires a urine pH < 6 and the presence of aspartic proteases. Patients with acute tubulointerstitial rejection had lower urine pH than stable transplants and healthy individuals. In addition, they had higher amounts of aspartic proteases and intact beta2-microglobulin in urine. These factors ultimately lead to increased amounts of cleaved urinary beta2-microglobulin. Cleaved beta2-microglobulin as an indicator of acute tubular injury may become a useful tool for noninvasive monitoring of renal allografts.

Sample characteristics

SpeciesTissue / SourceCompartmentDiseaseDetection method
Homo sapiensurinewholeTransplantationSELDI (H4 chip), MALDI

Sample description and preparation

Disease (case)acute renal allograft injury
Digestin-gel, trypsin
SeparationRP-HPLC

Molecule list

Molecule IDextExternal IDGeneNameRegulation (case/control)Scores
A1819XB2MG_HUMANB2M, HDCMA22PBeta-2-microglobulin precursorregulation in disease: upquantification method: SELDI signal intensity

Compile date 08-10-2018© iMODE-CKD consortium